New Suppliers

Sunrise Science Products

^MJSBioLynx is pleased to announce that we are now the Canadian distributor for Sunrise Science Products. Sunrise offers a wide variety of Yeast Growth Media. They offer complete media, as well as amino acid mixtures, dropout base, yeast nitrogen base and media components for S. cerevisiae and S. pombe. www.sunrisescience.com

New Products and Services

NuGEN - WT-Ovation™ Exon Module

The WT-Ovation™ Exon Module enables global gene expression analysis of small and degraded RNA samples such as FFPE, LCM, fine needle aspirates, sorted cells and blood on Affymetrix GeneChip® Exon and Gene ST arrays.

Assay Designs - Hsp27 EIA Kits

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHSP), which also includes alphaB-crystallin. Hsp27 acts as a molecular chaperone by inhibiting irreversible protein aggregation in response to physiological stresses such as heat shock or oxidative stress. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Macherey-Nagel - NucleoSpin® RNA XS

NucleoSpin RNA XS allows the isolation of highly concentrated and highly pure RNA from small samples. It is based on the reliable silica membrane technology, featuring the innovative NucleoSpin® XS column design which results in high RNA concentrations.

The innovative funnel shaped thrust ring of the NucleoSpin RNA XS holds a silica membrane of a very small diameter, allowing elution into only 5-20uL. Hence the NucleoSpin RNA XS is the ideal solution for extraction of RNA from trace amounts of sample giving the best results in sensitive downstream applications.

Micronic - 2D Coded and Non-Coded Black Tubes
For storing light sensitive samples

this catalogue features Assay Design's continually expanding product line which includes kits and materials for the detection and quantification of small molecules and proteins that are important in inflammation and immunity, oxidative stress, steroid and hormone biology, cell signaling, and bioenergetics. This catalogue features many Stressgen brand products.

Fall into savings with this buy 3, get 1 free promotion on Assay Designs top selling antibodies and proteins. All you need to do is select at least 4 Assay Designs™ antibodies or proteins from the list below and you will receive the lowest priced product free of charge.

Technical Report

Label-free Analysis of Microsatellite Instability in Colorectal Carcinoma by On-Chip Electrophoresis
By S.E. Baldus et. al

Abstract: Microsatellite instability (MSI) is caused by a failure of the DNA mismatch repair system and occurs frequently in various types of cancer. Since sporadic MSI are associated with approximately 10 to 15% of colorectal, gastric or endometrial carcinoma and impact clinical prognosis, MSI analysis is an important tool in clinical research and molecular diagnostics. Given that conventional techniques used for MSI detection - e.g. polyacrylamide gel electrophoresis (PAGE) or capillary electrophoresis - turned out to be laborious or expensive, this study aimed to develop a simple and efficient procedure of MSI detection. Forty cases were selected out of a panel of 150 patients with colon carcinoma. Of the forty cases selected, 26 cases had no MSI, while 14 cases had a positive MSI. Amplicons derived from areas of non-tumor (N) and tumor (T) were analyzed by microfluidic based on-chip electrophoresis on the Agilent 2100 bioanalyzer. The results presenting a pattern of five microsatellite loci were compared with the findings obtained by fluorochrome-associated PAGE technology. In all cases, label-free microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns for each of the five microsatellite loci. Detection of MSI was demonstrated by microsatellite loci-associated, well defined deviations in the electropherogram profiles of tumor and non-tumor material, in agreement with the classification of the MSI cases performed by conventional technology (95% concordance rate). The presented data demonstrate that microfluidic chip technology is a simple, reliable and robust technology for MSI detection, which allows label-free analyses of microsatellite amplicons within 30 minutes

Click here to read the full article

Announcement

NuGEN - Webinar: Exon Whole Transcriptome Profiling Using Small and Degraded RNA Samples

Date and Time: Wednesday, January 30, 2008 8:30 am Pacific Standard Time

Description: Global whole transcript and exon-based expression profiling is enabling biomarker discovery and novel target detection at a fundamental unit of gene expression—the exon. No longer limited to interrogation of just the 3’-most portion of a gene in global gene expression
studies, researchers are now discovering deeper and more biologically relevant information, and applying that knowledge to elucidating biological mechanisms in such important areas as disease/drug response and stem cell differentiation.

Click here to register.

Assay Designs - Upgrades to Endothelin-1 and TNF-a EIA Kits

Assay Designs new ET-1 EIA Kit reduces the assay time from overnight to 2 hours. It now consists of two monoclonal antibodies, requires less plate washing and offers more convenient incubation temperatures. In addition, standards have been recalibrated and reformatted from lyophilized to liquid.

The new TNF-a EIA Kits offer a 35 fold increase in sensitivity, detecting lower levels of TNF-a. All incubations are done at room temperature and the flexible format comes with break-a-part plate strips which allow for creative experimental design.