e-Lynx - January 2009Dear Researcher, We are pleased to present the January e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions
ExonHit - Rat Genome SpliceArray™
ExonHit’s SpliceArray™ microarrays constitute a novel type of microarray designed to provide high resolution gene expression profiling by generating gene expression data on both a gene and splice isoform level within the same microarray. By combining their patented approach with Agilent Techologies and Affymetrix microarray based platforms, they have created a workflow solution that yields high quality data. Contact our Technical Support Department for more information.
Trevigen - CometAssay™ Electrophoresis System
Click here to view the CometAssay Electrophoresis System Manual.
StressMarq - IHC Assay Kits
Kits are available for DNA
Damage, Nitrotyrosine, CamKII, HSP70 and HSP90. Image: Anti-Hsp70. The human colon tissue stained (brown) mostly in inflammatory cells. Shown at a 1:10,000 dilution of SKC-240 using the ABC method.
Macherey-Nagel
- Protein Quantification Assay
|
ExonHit
now offers SpliceArray™
for use in studying Rat models of Human disease and
toxicogenomic applications. SpliceArray™
products provide the highest resolution expression profiling
commercially available today. In addition to gene expression
profiling, the Rat SpliceArray™ will allow researchers
to identify novel transcript isoforms that may be involved
in response to therapeutic compounds for better understanding
of drug discovery, toxicity pathways and mechanism of
action safety issues. This unique approach can save
months of discovery and pre-clinical development time.
Stressmarq’s
Immunohistochemistry assays utilizes novel licensed technology
aimed at removing limitations associated with the antibody
staining of frozen and paraffin embedded tissue sections.
The technology includes specific buffer formulations for
antigen exposure, and optimized antibody formulation. It
also includes a unique container called the Antibody Amplifier™.
Incubating slides in antibody solution while rocking on
a standard laboratory rocker guarantees even staining, and
reproducible results. 
| Product Number | Description | Pkg Size |
| MCN74096750 | Protein Quantification Assay | 50 Assays (+ 6 calibration curves) |
| MCN740967250 | Protein Quantification Assay | 250 Assays (+25 calibration curves) |
Abgent
- Biotin Single Shot Labeling Kit
Abgent's Biotin Single-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per antibody molecule.
Click here to view the Biotin Single Shot Labeling kit Manual.
| Product Number | Description | Pkg Size |
| ABGKT1001A | Biotin Single Shot Labeling Kit | Kit |
New Literature
Polyplus
Transfection - News 5
This issue of Polyplus Transfection News includes a review of transfection for recombinant protein and virus production. Click here to download a copy.
Meridian
Life Sciences - Antibodies, Antigens, Infectious Diseases
& Toxins Catalogue
This electronic catalogue includes over 5500 antibodies, antigens and immunoassay reagents, the new recombinant proteins from yeast and much more. Click here to download your copy.
Promotions
AthenaES®
- Special Introductory Trial Pricing and Free
T-Shirt Offer
Athena's proprietary Expression Media are world renowned for their reliability and superior performance in the production of recombinant proteins. For a limited time, take advantage of special pricing on the following items:
- Animal Product Free Media Optimization Kit
- FNC Coating Mix® - complete serum-free medium; increases attachment of cells
- Atholate™ Packets - proprietary blend
of plant protein hydrolysates that are APF Certified™
and match the performance and nutritional characteristics of
standard casein hydrolysate
The first 10 customers who order AthenaES® products from MJSBioLynx will receive a free AthenaES® T-Shirt.
Click here for promotion detail
Offer valid until March 31, 2009 or while quantities
last
Cannot be combined with any other offers or discounts
Greiner
Bio-One - Free Pizza Party Promotion is Back!
For
the cold Canadian winter months of January to
March 2009 - enjoy hot, delicious pizza on us!
Our thank you to our wonderful MJSBioLynx
customers.
Accumulate $500 in
Purchases of Greiner Bio-One Products from MJSBioLynx
and Receive a $50 Pizza Gift Certificate.
Click here for more information.
Greiner Bio-One offers high quality labware such as cell culture labware, microplates and tubes.
Limit 2 Pizza Parties
per Lab
Cannot be combined with other offers
Please refer to promotional code GR1309
Assay Designs - 50% OFF an ImmunoSet Buffer Pack with the Purchase of Any ImmunoSet
The
Assay Designs ImmunoSet Buffer Pack provides an
all-purpose buffer that is easily modified to
coat, block, and wash plates in addition to diluting
standards and samples. TMB substrate and acid
stop solution for color development and reaction
termination are also supplied.
This offer is valid until April 30th, 2009
Click here for details.
USB
- Spend $150 on USB Biochemicals and
we'll send you the USB fitness pack Free!
USB
provides biochemicals that deliver reliable and
consistent results. USB biochemicals and Ultrapure
brand products meet the highest industry standards
for quality. They are available in a variety of
grades such as ACS Reagent Grade, CP Grade (Chemically
Pure), USP Grade (USBioAnalyzed) and Molecular
Biology Grade.
Don't forget about their line of convenience reagents. USB offers an extensive selection of Ultrapure biochemical reagents in premixed, ready-to-use formats for convenience in any lab.
Now's a great time to step forward and purchase USB Biochemicals and convenience reagents. When you spend $150 or more, we'll send you a USB water bottle and pedometer to track your own fitness results.
This offer is available
while quantities last
Please reference promotional code 28H-2300W
Click here for promotion details.
Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand
ChIP-IT™
Express HT allows you to perform chromatin IP
in a fast, reproducible high-throughput format.
It combines the efficiency of the groundbreaking
magnetic bead-based ChIP-IT™ Express Kit
with a 96-well plate format, enabling the rapid
and efficient processing of a large number of
ChIP reactions.
This offer is available while quantities last
Click here for promotion details
Technical Report
The Selection of Optimum
Media Formulations for Improved Expression of Recombinant
Proteins in E. coli.
by Sheldon E. Broedel
et al.
Escherichia coli is routinely
used for the production of recombinant proteins both for
research purposes and for commercial applications. Despite
the extensive amount of knowledge gathered about the synthesis
and accumulation of heterologous proteins and the well established
principals
affecting the production of secondary metabolites, little
attention has been paid to the effects of medium composition
on the production of recombinant proteins. In the course
of optimizing the production of various recombinant proteins,
we found that the level of accumulation of a given protein
was composition-dependent. Since the best formulation for
a protein could not be determined solely on the basis of
the class of protein, a simple screening technique was developed
to rapidly identify the best available medium for any give
recombinant protein. The screen was tested on six different
recombinant proteins and shown to be an effective means
of empirically determining the best medium. It was further
observed that the medium composition affected the relative
level of soluble protein that accumulated. These results
suggested that a medium screen should be employed as a routine
part of developing bacterial strains which produce recombinant
proteins. The application of such a screen could aid in
improving the recovery of recombinant proteins.
Click here to read the full article.
Tradeshows and Events
| Event | Location | Date |
| Minishow
Note: Refreshments will be available. |
University of Guelph Science Complex Room 4440 |
January 14, 2009 |
Announcements
NuGEN - Agilent Solution
Good News for Agilent Microarray
Users !
Now you can interrogate small and degraded samples from a wide variety of sources including FFPE, whole blood, LCM, fine needle aspirates, tissue biopsies, sorted cells, and more, and successfully label sample inputs as low as 500 picograms—up to 100-fold less than current available target preparation methods.
NuGEN is currently in the pre-release testing phase of an improved Cy3/Cy5 labeling system for labeling cDNA targets amplified using WT-Ovation™ kits—both the Pico or FFPE modules—for interrogation on Agilent Dual-Mode Gene Expression DNA microarrays.
Now Agilent microarray users can tap into the valuable archives of well-annotated samples that were previously out of reach for inquiry.
Visit the NuGEN Inc. Website for more information, or contact our Technical Support Department.
MJSBioLynx - New Sales Representative - Quebec and New Brunswick
MJSBioLynx
is pleased to announce that Ms. Édith Beaulieu
has been appointed as the Technical Sales Representative
for the provinces of Quebec and New Brunswick. She
will be pleased to answer your technical questions
on our products, to present you with the newest
technologies on the market or to receive your comments
regarding our company.
Ms. Beaulieu completed her Ph.D and her M.Sc. in physiology at the Université de Montréal as well as a B.Sc in Biochemistry at the Université du Québec à Montréal. She has gained valuable knowledge through her work experience in molecular oncology laboratories.
MJSBioLynx is proud to
have Édith join our team. She can be contacted
at our toll free number, 1-888-593-5969 x 403 or
by e-mail, edithb@biolynx.ca.
Assay Designs
now offers Abnova Antibodies
Assay Designs now offers over 3100 highly characterized monoclonal antibodies from Abnova. These antibodies are certified for applications such as ELISA, Immunohistochemistry, Immunofluorescence and Western Blotting.
Visit the Assay Designs Website for product information.
