e-Lynx - June 2008Dear Researcher, We are pleased to present the June e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.
New Products and ServicesMacherey-Nagel
- NucleoSpin® RNA Clean-up XS
|
| Product Number | Description | Pkg Size |
| MCN74090310 | NucleoSpin® RNA Clean-up XS | 10 Preps |
| MCN74090350 | NucleoSpin® RNA Clean-up XS | 50 Preps |
| MCN740903250 | NucleoSpin® RNA Clean-up XS | 250 Preps |
Abgent
- Antibody Sampler Kits
Sampler kits offer an affordable way to purchase multiple validated antibodies from Abgent’s comprehensive antibody product lines. Assemble a collection of antibodies to your pathway of interest, or economically screen for the antibody that is perfect for your application.
Click on the product descriptions
in the table below for a list of qualified antibodies for
each sampler kit. Choose a minimum of 5 antibodies per order.
| Product Number | Description | Pkg Size |
| ABGAS1000A | Apoptosis Sampler Kit | Kit |
| ABGAS1001A | Autophagy Sampler Kit | Kit |
| ABGAS1002A | Acetylation Sampler Kit | Kit |
| ABGAS1003A | Methylation Sampler Kit | Kit |
| ABGAS1004A | Neuroscience Sampler Kit | Kit |
| ABGAS1005A | Phosphospecific Sampler Kit | Kit |
| ABGAS1006A | Stem Cell Sampler Kit | Kit |
| ABGAS1007A | Sumoylation Sampler Kit | Kit |
| ABGAS1008A | Ubiquitination Sampler Kit | Kit |
Trevigen - HT Fluorescent Homogeneous PARP Inhibition Assay
Trevigen's Homogeneous PARP Inhibition Assay is a highly sensitive fluorescent screening assay for the rapid identification of PARP-1 inhibitors in an in vitro system. This one hour endpoint assay is performed in two successive steps requiring only the consecutive addition of reaction components. In addition, the assay can be used to determine relative IC50 values for PARP inhibitors and is capable of detecting as little as 10% inhibition of PARP-1 activity. The Homogeneous PARP Inhibition Assay is designed for the screening of PARP inhibitors using a NAD concentration below the Km of PARP. Once potential inhibitors are identified, results can be confirmed using Trevigen's HT Universal PARP Assay kits (Product # TV4676096K or TV4677096K).
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Fluorescent, non-radioactive format
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High throughput 96 test size
-
Detects as little as 10% inhibition of PARP-1 activity
| Product Number | Description | Pkg Size |
| TV4690096K | HT F Homogeneous PARP Inhibition Assay | 96 tests |
Active Motif - MAX Stain™ Universal Immunofluorescence System
MAX
Stain makes immunofluorescence experiments simpler and more
consistent by providing you with a complete set of proven,
QC-tested components and an optimized protocol. All you
need to supply is a cell line and a primary antibody directed
against your target protein. Active Motif's popular Chromeo™
488 Goat anti-Rabbit IgG is included as it is a commonly
used secondary antibody. Other Chromeo secondary antibodies,
and those from other sources, can also be used.
Figure1: Staining
with the MAX Stain Universal Immunofluorescence System.
Bovine pulmonary artery endothelial cells were labeled
for mitochondria (red), F-actin (green) and DAPI (blue)
using the MAX Stain Universal Immunofluorescence System.
| Product Number | Description | Pkg Size |
| AM15250 | MAX Stain™ Universal Immunofluorescence System | Kit |
Heathrow
- Rota-Filler™ 5000
-
Accommodates pipets ranging from 1 to 100 mL
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Comes with two extra hydrophobic 0.45µm filters that fit in the autoclavable polypropylene nozzle.
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Adjustable
pivot arm rotates to five positions: 0°, 5°, 10°,
15°, and 20° - allows for more flexibility especially
when working in restricted spaces; such as fume-hoods or clean
cabinets. -
Liquid crystal display (LCD) with easy-to-read battery charge levels as well as speed gravity settings.
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Includes a rechargeable NiMH battery pack that provides 8 hours of continuous operation and is quick to recharge.
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Adjustable speed dial provides improved control with a wider range of settings: faster maximum speeds and slower minimum speeds - allows for finer aspirate dispense control. Can also set to gravity mode to dispense “to contain” pipets.
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Ergonomically designed for end-user comfort
-
Filler measures 160 x 40 x 150 mm.
| Product Number | Description | Pkg Size |
| HS5000RFU | Rota-Filler™ 5000 with Power Adapter | Each |
Active
Motif - Universal Magnetic Co-IP Kit
Magnetic beads for low background co-immunoprecipitation
The
Universal Magnetic Co-IP Kit improves co-immunoprecipitation
(Co-IP) through the use of protein G-coated magnetic
beads, which speed and simplify the procedure while
greatly reducing background. The kit includes optimized
reagents for making both nuclear & whole-cell extracts
from cells or tissue, giving you the flexibility to
Co-IP any protein complex, whether it was originally
bound to DNA or in the cytoplasm.
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Perform Co-IP of nuclear or cytoplasmic protein complexes, beginning from cells or tissue
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Magnetic beads speed and simplify the procedure while reducing background, enabling the use of low-stringency buffers that maintain complex integrity
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Nuclear extraction procedure removes nuclear protein complexes from the DNA without disrupting protein/protein interactions
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Inhibitor solutions included to preserve the integrity of protein modifications
| Product Number | Description | Pkg Size |
| AM54002 | Universal Magnetic Co-IP Kit | 25 rxns |
USB
- HotStart-IT® qRT-PCR Master Mix Kits
High specificity, sensitivity and consistent
performance
The new qRT-PCR Master Mixes are based on HotStart-IT® technology using a primer sequestration method which increases specificity, prevents primer-dimer formation and has no extensive heating step.
The HotStart-IT® one-step qRT-PCR Master Mix kits provide optimal performance and maximum convenience for real-time quantitative analysis of RNA templates in a single reaction format. Both SYBR® green and probe based kits are available.
Click here
for promotion information
Click on image for hi-res PDF version
| Product Number | Description | Pkg Size |
| UB75770S1 | HotStart-IT® SYBR® Green One-Step qRT-PCR Master Mix Kit | 100 rxns |
| UB75770S2 | HotStart-IT® SYBR® Green One-Step qRT-PCR Master Mix Kit | 500 rxns |
| UB75772S1 | HotStart-IT® Probe One-Step qRT-PCR Master Mix Kit | 100 rxns |
| UB75772S2 | HotStart-IT® Probe One-Step qRT-PCR Master Mix Kit | 500 rxns |
Assay
Designs - Creatinine and Formaldehyde Detection Kits
Creatinine Colourimetric Detection Kit
Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo this conversion appears to be irreversible. Under normal conditions, creatinine formation occurs at a relatively constant rate. Intra-individual variation of creatinine is < 15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.
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Easy-to-use - Safer single-step detection reagent is ready-to-use out of the box
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Sensitive - Detect as low as 0.019 mg/dL
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Reproducible - Inter- and intra-assay precision < 4% (n = 20)
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Fast - Homogeneous mix-and-read assay with results in just 30 minutes
-
Accurate - Standard is calibrated to a NIST creatinine standard
Formaldehyde Fluorometric Detection Kit
Formaldehyde is a colourless, flammable, strong-smelling gas that can undergo rapid chemical changes immediately after absorption. Exposure occurs primarily by inhaling formaldehyde gas or vapor from the air or by absorbing liquids containing formaldehyde through the skin. Studies have suggested that formaldehyde may affect the lymphatic and blood systems and that exposure to formaldehyde may cause leukemia, particularly myeloid leukemia, in humans.
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Easy-to-use - Simple homogeneous mix-and-read assay with no wash steps
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Sensitive - Accurately measure as little as 0.775 µM formaldehyde
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Reproducible - Inter- and intra-assay precision < 8% (n=20)
-
Fast - Save time with results in just 30 minutes
| Product Number | Description | Standard Range | Sensitivity | Sample Type | Time to Answer | Species Reactivity | Pkg Size |
| ADI907030 | Creatinine Colourimetric Detection Kit | 0.31-20 mg/dL | 0.019 mg/dL | Urine | 30 min | Species Independent | 2 x 96 wells |
| ADI907031 | Formaldehyde Fluorometric Detection Kit | 3.125-200µM | 0.775µM | Urine, Culture Supernates | 30 min | Species Independent | 2 x 96 wells |
CryoBioPhysica - pIsep pH Gradient Chromatography System
pIsep
is a mobile phase system which enables fully controllable
linear and nonlinear pH gradients and dual independent
gradients (pH & NaCl, etc). pIsep combines the resolution
of isoelectric focusing with the capacity and automation
of HPLC. pIsep buffers are volatile, so you can use
LC/MS detection to its fullest or retrieve proteins
from preparative samples by evaporation alone.
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Applicable to analytical and large scale preparative purifications of proteins
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Separates isoforms of immunoglobulins (even monoclonal antibodies possessing heterogeneously charged sugar chains)
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Separate protein isoforms differing by a single amino acid or species with variations in glycosylation.
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Can replace salt-elution Ion Exchange Chromatography. Reduces separation steps and eliminates all of the desalting steps associated with with salt gradient chromatography
-
Can be used as a polishing step following: Affinity chromatography, Hydrophobic interaction chromatography, Reversed phase chromatography, Ion-exchange chromatography, Gel filtration chromatography
-
Can be used in quality control - pIsep can detect degradation or folding variants of proteins.
| Product Number | Description | Pkg Size |
| PI801S | pIsep pH Gradient Maker Software
(1 Manifold - NaCl default) |
each |
| PI802P | pIsep pH Gradient Maker Pro Software (3 Manifolds - NaCl, Urea, Acetonitrile) |
each |
| PI803 | pIsep Buffer Kit | 5L Preparation |
| PI804 | pIsep Buffer Kit | 25L Preparation |
| PI805 | pIsep Buffer Kit | 50 L Preparation |
| PI806 | pIsep Buffer Kit | 100 L Preparation |
Click here for more information
New Literature
Active
Motif - Motifvations Newsletter
This issue of Motifvations features the new Suspension Cell FACE™ Kits for quantifying phospho-proteins, HDAC assay kits, MAX Stain™ and the new magnetic Co-IP kits.
Simport
- Micrewtube Brochure
This brochure features the Micrewtube family of products. Featuring the new Micrewlock™ tubes with tamperproof cap, Simport offers a tube for every application
Promotions
Active Motif - Free Gifts or 20% Discount with the Purchase of ChIP-IT™ Kits
ChIP-IT Kits - Making successful chromatin immunoprecipitation fast and easy
Buy
1 ChIP-IT™ Kit
and receive a FREE box of Greiner Bio-One 10 mL Serological
Pipettes
Buy 2 ChIP-IT™ Kits
and receive a FREE MJSBioLynx StripFuge
mini centrifuge
Buy 3 ChIP-IT™ Kits
and receive a FREE package of Ready-to-ChIP HeLa Chromatin
OR
Get a 20% Discount
on your order of ChIP-IT™ kits and related products
Click here for promotion details
Please reference promotion
code AMC08 at time of order
Offer valid until July 31st, 2008
Cannot be combined with any other offers or discounts
USB - Promotional Offers
1 - Save 50% OFF
the New HotStart-IT® qRT-PCR Master Mixes
For a limited time, take
advantage of special pricing on both USB HotStart-IT®
qRT-PCR Master Mix Kits in the 100 reaction format.
These are available as SYBR® Green and Probe based
kits.
2 - Save $25 on any order of USB products of $250 and Up!
Click here for more details
Offers valid until August
31st, 2008
Please reference promotional code HS08
Cannot be combined with any other offers or discounts
Simport
- 25% OFF LockMailer™ Microscope Slide Jar
Get a 25% Discount on Simports
new LockMailer™ Jars,
tamperproof multi purpose containers for mailing, staining
or storing microscope slides.
Click here for promotion details
Offer valid until October
31st, 2008
Cannot be combined with any other offers or discounts
Please reference promotional code SP0410
Elchrom
- Origins Electrophoresis Bundle Promotion
Purchase an Elchrom Origins
Electrophoresis Unit
and receive the following items FREE of charge
- Easy Stain Gel Tray
-
Peel-IT™
- For easy removal of gel backing -
Your choice of Nucleic Acid Electrophoresis Gel Starter Packs
Click here for promotion details
Offer valid until July 31st, 2008
Please reference promotional code EL0508
-
Anti-Nitrotyrosine Antibody, Clone39B6
-
Anti-DNA Damange Antibody, Clone15A3
-
Anti-PSD95 Antibody, Clone 6G6
Click here for promotion details
Promotion valid until July
31st, 2008
Cannot be combined with any other offers or discounts
Please refer to promotional code SM0408
Macherey-Nagel
- iPod® Promotion
Until June 30,
2008
Take advantage of special pricing on
NucleoSpin® Kit Bundles
AND receive a FREE iPod nano
Click
here for details
Offer valid from March
1st to June 30th, 2008
Cannot be combined with any other offers
or discounts
Promotional Code MCNI08
Abgent - 40% OFF Newly Released Antibodies
Abgent strives to provide you with an expansive and unique antibody collection for advancing your research.
Abgent now offers huge savings
on new products.
Click here
for a listing of applicable products and promotion details
Assay
Designs - Buy 3, Get 1 Free - Antibody and Protein Promotion
Offer extended until December 31st, 2008
Fall into savings with this buy 3, get 1 free promotion on Assay Designs top selling antibodies and proteins. All you need to do is select at least 4 Assay Designs™ antibodies or proteins from the list below and you will receive the lowest priced product free of charge.
-
Over 110 antibodies and proteins to choose from, including many Stressgen® brand products
-
Diverse set of research areas including heat shock, cell signaling, oxidative stress, and inflammation
-
Superb performance documented in thousands of peer-reviewed publications
Click here for promotion details
Valid until December 31st,
2008 or while quantities last
Promotional Code: EOY112607
Cannot be combined with any other offers or discounts
Technical Report
Comparison of
Matched Lung FFPE and Fresh Frozen Expression Profiles
Using the WT-Ovation™ FFPE System
by Nugen Technologies
Inc.
Generation of expression signatures from FFPE-derived RNA has been historically challenging due to the extreme conditions involved in specimen handling and the tissue fixation process which typically result in cross-linking and degradation of nucleic acids isolated from these tissues. The NuGEN WT-Ovation™ FFPE System overcomes these challenges by allowing researchers to amplify from limited amounts of significantly degraded RNA.
Click here to read the full article.
Tradeshows
Visit MJSBioLynx at the following events:
| Tradeshow | Location | Date |
| RiboWest | University of Lethbridge | June 16 and 17, 2008 |
| Minishow - UBC | University of British Columbia | June 13, 2008 11:00 am to 1:00 pm |
