e-Lynx - June 2009

Dear Researcher,

We are pleased to present the June e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.

New Products

NuGEN - Applause™ Systems: a practical solution for today's RNA amplification challenges

The Applause products offer NuGEN’s proven Single Primer Isothermal Amplification (SPIA®) technology for analyzing RNA samples of 50 ng and up. Powerful and cost effective, Applause delivers a simpler, faster and more practical solution to everyday microarray target preparation needs.
  • Practical: Amplify total RNA samples starting at 50 ng
  • Fast: RNA to array in a single day
  • Simple: Single-tube, add—and— incubate assay
  • Proven: Based on SPIA linear amplification technology
  • Cost-effective: Ideal for everyday amplification
NuGEN’s first products in the Applause line are the WT-Amp ST and WT-Amp Plus ST Systems designed for preparing targets for interrogation on the Affymetrix GeneChip® Gene ST and Exon ST arrays, respectively.

Click here for additional product information.

Product Number
Description
Pkg Size

NU550024

Applause WT-Amp ST System

24 rxns
NU551024 Applause WT-Amp Plus ST System 24 rxns

Labnet - MPS 1000 Mini Plate Spinner

  • Quickly Spin down droplets and condensation
  • Use before and after thermal cycling to increase PCR yield
  • Accepts skirted, non-skirted and all standard PCR plates
  • Less than 1/4 the size of most plate centrifuges
Click here for more information.

 

Product Number
Description
Pkg Size

LNC1000

MPS 1000 Mini Plate Spinner Centrifuge

Each

 

Micronic - SampleScan Mini Barcode Reader
A smaller, faster 2D Barcode-scanning solution

  • Scans 96 tubes in 5-7 seconds
  • Compatible with SBS racks in 12, 24, 48 and 96 well configurations
  • Built-in auto rescan feature
  • Single tube read function
  • Manually edit barcodes
  • Customizable output files and destination folder (.csv)
  • LIMS-integration ready
  • ActiveX SDK controls available to assist with robotics and LIMS integration

 

Product Number
Description
Pkg Size

MSMP87000

SampleScan Mini Reader

Each


Promotions

Macherey-Nagel - 25% OFF Bioanalysis Products During the Month of June 2009

Macherey-Nagel offers quality products for the purification of:
  • Plasmid DNA
  • PCR Products and DNA Fragments
  • RNA
  • gDNA from blood, tissue, plants, forensic samples, etc.
  • Viral DNA and RNA
  • Proteins

Click here for promotion details.

 

Greiner Bio-One - Value Added Offers

Until June 30th, 2009
Take advantage of 3 value added offers on Greiner Bio-One Labware
  • Buy 3 cases of CELLSTAR® HTS plates, Get 1 case free (of same item)
  • Buy 2 cases of ELISA plates, get a $25 gift certificate
  • Buy 2 cases of flasks, get a free case of cell scrapers

Click here for promotion details.

Greiner Bio-One - Buy 3 Cases of 15 or 50 mL Centrifuge Tubes and Get 1 Case FREE

Mix and Match!
Until July 31st, 2009,
Get a FREE case* of centrifuge tubes
after every 3 cases purchased.

Click here for promotion details.
* Free case of equal or lesser value.

 

Platypus Technologies - 25% Discount on the Oris™ Cell Migration Assay - TriCoated Kit

  • Utilizes patent pending, cell seeding stoppers to create a 2 mm detection zone in the center of each well.
  • Compare three surfaces for optimal cell migration on the same plate (1) Tissue Culture treated, 2) Collagen I coated, and 3) Fibronectin coated ).

Click here for promotion details

Micronic - Get the SampleWare Software for only $130 when you purchase a Micronic Scanner, Reader or StarterPack!

Improve your laboratory's efficiency with SampleWare - laboratory management software that allows you to store, organize and track your sample data.

Click here for promotion details.

 

Polyplus Transfection - jetPRIME™ Samples - The new DNA and siRNA transfection reagent

  • Outstanding efficiency with extremely low toxicity
  • Uses low amounts of nucleic acid - only 2 µg of DNA per well in 6-well plates
  • Efficient transfection into mammalian cell lines such as HeLa, HEK-293 and CHO cells
  • Very Convenient: No medium changes required, compatible with serum and antibiotics
Contact our Technical Support Team to inquire about obtaining a FREE sample of jetPRIME™.

This offer is valid while quantities last.

Abgent - Free Chocolate with the Purchase of Antibodies

From April 1st to June 30th, 2009
Accumulate purchases of 5 Abgent antibodies* and receive a $50 gift card for Laura Secord Chocolates.
Abgent antibodies are widely used in the fields of :
  • Autophagy
  • Apoptosis
  • Cell Function
  • Gene Regulation
  • Stem Cells
  • Phosphorylation

Click here for details.
* Offer is not applicable to Abgent Antibody Sampler Kits.

Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand

ChIP-IT™ Express HT allows you to perform chromatin IP in a fast, reproducible high-throughput format. It combines the efficiency of the groundbreaking magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate format, enabling the rapid and efficient processing of a large number of ChIP reactions.
This offer is available while quantities last
Click here for promotion details

Technical Report

The Use of ImageJ to Quantify Cell Number in the Oris™ Cell Migration Assay
by: Scott R. Gehler, Ph.D., Platypus Technologies, LLC

Introduction
Cell migration is critical for many physiological events, including embryonic development, wound healing, and the inflammatory response. Furthermore, aberrant motile behavior of cells contributes to pathological processes including tumor metastasis and arthritis (1).
The Oris™ Cell Migration Assay (Figure 1) uses a 96-well plate populated with silicone stoppers that exclude cells from the central Detection Zone of the well. After cells are seeded and allowed to adhere, the silicone stoppers are removed to reveal a 2mm diameter unseeded region in the center of each well, into which cells are permitted to migrate.
ImageJ is a freeware image analysis program developed at the National Institutes of Health (http://rsb.info.nih.gov/ij). This application note describes a method to measure cell migration, using ImageJ, by counting the number of cells that have migrated into the Detection Zone in an Oris™ Cell Migration Assay.
Click here to read the complete article.

Events

Tradeshows
Meet MJSBioLynx at the following events:

Tradeshow Location Date

Atlantic Omics Symposium
Including a seminar presented by NuGEN Technologies Inc.
see details below.

Delta Beauséjour
Moncton, NB
June 18-19, 2009

Seminar: Ribo-SPIA Based RNA Amplification System for Use with Lysate from One or More Cells for Global Gene Expression Analysis

Presented by: Mark Hurt, Senior Field Application Scientist, NuGEN Technologies Inc.

Abstract: Until now, researchers have been unable to perform global gene expression analysis from a single cell using microarray technology. The WT-Ovation(tm) One-Direct RNA Amplification System makes possible microarray analysis from one cell, or just a few cells, while eliminating costly, time-consuming RNA purification. Biological samples are composed of heterogeneous cell populations. Therefore, determining the functional contribution of each individual cell in such a complex matrix requires the granularity of a true single-cell interrogation methodology. The WT-Ovation One-Direct System is a breakthrough for researchers developing more effective diagnostic tools and targeted drug therapies in immunology, neurobiology, hematopoiesis, and cancer stem cell biology. We will present array data generated using this amplification technology on a number test systems and cell types.

Announcements

Greiner Bio-One Goes Green!

Greiner has added several environmentally friendly features to their products and packaging. Click here to read more about their green initiatives.
Greiner Bio-One produces high quality labware such as tissue culture labware, microplates, serological pipettes and tubes.

Labnet - MultiGene Thermal Cyclers and Hermle Centrifuges are Now Available from MJSBioLynx Inc.

We are pleased to announce that the following products are now available from MJSBioLynx Inc:

- MultiGene Gradient Thermal Cycler - Produces consistent, reliable results while making gradient technology affordable. This unit is extremely simple to program, compact in design and built to perform.

- MultiGene II Personal Thermal Cycler - Combines a versatile and precise cycling unit with easy to use software. Not limited to the amplification of nucleic acids, the unit is also useful for applications such as enzymatic digestion, ligation, and other procedures that require incubation of small samples in a temperature controlled environment.

- Hermle Centrifuges - We are pleased to re-introduce the Hermle Line of Centrifuges. This product line offers a wide variety of centrifuges, rotors and accessories to meet the needs of your particular applications.

Labnet offers quality products for centrifugation, liquid handling, as well as shakers and rockers, constant temperature equipment, molecular biology equipment and laboratory plastics.

Please contact our Technical Support Team for more information on these products or to request a Labnet catalogue.

Tech Support Link
 
1-888-593-5969
P.O. Bag 1150, 300 Laurier Blvd., Brockville, Ontario, Canada, K6V 5W1, Fax: (613) 342-1341

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