e-Lynx - June 2009
Dear Researcher,
We are pleased to present the June
e-Lynx, a newsletter devoted to keeping our customers up-to -date
on new technologies, our new products, suppliers, activities and
promotions.
NuGEN - Applause™ Systems: a practical
solution for today's RNA amplification challenges
The Applause products offer NuGEN’s
proven Single Primer Isothermal Amplification (SPIA®) technology
for analyzing RNA samples of 50 ng and up. Powerful and cost effective,
Applause delivers a simpler, faster and more practical solution
to everyday microarray target preparation needs.
-
Practical: Amplify total RNA
samples starting at 50 ng
-
Fast: RNA to array in a single
day
-
Simple: Single-tube, add—and—
incubate assay
-
Proven: Based on SPIA linear
amplification technology
-
Cost-effective: Ideal for everyday
amplification
NuGEN’s first products in the
Applause line are the WT-Amp ST and WT-Amp Plus ST Systems designed
for preparing targets for interrogation on the Affymetrix GeneChip®
Gene ST and Exon ST arrays, respectively.
Product
Number |
Description |
Pkg
Size |
NU550024 |
Applause WT-Amp ST System |
24 rxns |
| NU551024 |
Applause WT-Amp Plus ST System |
24 rxns |
Labnet
- MPS 1000 Mini Plate Spinner
-
Quickly Spin down droplets and
condensation
-
Use before and after thermal
cycling to increase PCR yield
-
Accepts skirted, non-skirted
and all standard PCR plates
-
Less than 1/4 the size of most
plate centrifuges
Product
Number |
Description |
Pkg
Size |
| LNC1000 |
MPS 1000 Mini Plate Spinner Centrifuge |
Each |
Micronic
- SampleScan Mini Barcode Reader
A smaller, faster 2D Barcode-scanning solution
-
Scans 96 tubes in 5-7 seconds
- Compatible with SBS racks in 12, 24, 48 and 96
well configurations
- Built-in auto rescan feature
- Single tube read function
- Manually edit barcodes
- Customizable output files and destination folder
(.csv)
- LIMS-integration ready
- ActiveX SDK controls available to assist with
robotics and LIMS integration
Product
Number |
Description |
Pkg
Size |
| MSMP87000 |
SampleScan Mini Reader |
Each |
Macherey-Nagel
- 25% OFF Bioanalysis Products During the Month of June 2009
Macherey-Nagel offers quality products
for the purification of:
Greiner
Bio-One - Value Added Offers
Until June 30th, 2009
Take advantage of 3 value added offers on Greiner Bio-One Labware
-
Buy 3 cases of CELLSTAR®
HTS plates, Get 1 case free (of same item)
- Buy 2 cases of ELISA plates, get a $25 gift certificate
- Buy 2 cases of flasks, get a free case of cell
scrapers
Click
here for promotion details.
Greiner
Bio-One - Buy 3 Cases of 15 or 50 mL Centrifuge Tubes and Get
1 Case FREE
Mix
and Match!
Until July 31st, 2009,
Get a FREE case* of centrifuge tubes
after every 3 cases purchased.
Click
here for promotion details.
* Free case of equal or lesser value.
Platypus
Technologies - 25% Discount on the Oris™ Cell Migration
Assay - TriCoated Kit
-
Utilizes patent pending, cell
seeding stoppers to create a 2 mm detection zone in the center
of each well.
-
Compare three surfaces for optimal
cell migration on the same plate (1) Tissue Culture treated,
2) Collagen I coated, and 3) Fibronectin coated ).
Micronic
- Get the SampleWare Software for only $130 when you purchase
a Micronic Scanner, Reader or StarterPack!
Improve your laboratory's efficiency
with SampleWare - laboratory management software that allows you
to store, organize and track your sample data.
Polyplus
Transfection - jetPRIME™ Samples - The new DNA and siRNA
transfection reagent
This offer is valid while quantities last.
Abgent -
Free Chocolate with the Purchase of Antibodies
From
April 1st to June 30th, 2009
Accumulate purchases of 5 Abgent antibodies* and receive a $50 gift
card for Laura Secord Chocolates.
Abgent antibodies are widely used
in the fields of :
- Autophagy
- Apoptosis
- Cell Function
|
- Gene Regulation
- Stem Cells
- Phosphorylation
|
Click
here for details.
* Offer is not applicable to Abgent Antibody Sampler Kits.
Active
Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96
Magnetic Stand
ChIP-IT™
Express HT allows you to perform chromatin IP in a fast, reproducible
high-throughput format. It combines the efficiency of the groundbreaking
magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate
format, enabling the rapid and efficient processing of a large number
of ChIP reactions.
This offer is available while quantities
last
Click
here for promotion details
The Use of ImageJ to Quantify
Cell Number in the Oris™ Cell Migration Assay
by: Scott R. Gehler, Ph.D., Platypus
Technologies, LLC
Introduction
Cell migration is critical for many physiological events, including
embryonic development, wound healing, and the inflammatory response.
Furthermore, aberrant motile behavior of cells contributes to pathological
processes including tumor metastasis and arthritis (1).
The Oris™ Cell Migration Assay
(Figure 1) uses a 96-well plate populated with silicone stoppers
that exclude cells from the central Detection Zone of the well.
After cells are seeded and allowed to adhere, the silicone stoppers
are removed to reveal a 2mm diameter unseeded region in the center
of each well, into which cells are permitted to migrate.
ImageJ is a freeware image analysis
program developed at the National Institutes of Health (http://rsb.info.nih.gov/ij).
This application note describes a method to measure cell migration,
using ImageJ, by counting the number of cells that have migrated
into the Detection Zone in an Oris™ Cell Migration Assay.
Tradeshows
Meet MJSBioLynx at the following
events:
| Tradeshow |
Location |
Date |
| Atlantic
Omics Symposium
Including a seminar presented by NuGEN Technologies Inc.
see details below. |
Delta Beauséjour
Moncton, NB |
June 18-19, 2009 |
| Seminar:
Ribo-SPIA Based RNA Amplification System for Use with Lysate
from One or More Cells for Global Gene Expression Analysis
Presented by: Mark Hurt,
Senior Field Application Scientist, NuGEN Technologies Inc.
Abstract: Until now, researchers
have been unable to perform global gene expression analysis
from a single cell using microarray technology. The WT-Ovation(tm)
One-Direct RNA Amplification System makes possible microarray
analysis from one cell, or just a few cells, while eliminating
costly, time-consuming RNA purification. Biological samples
are composed of heterogeneous cell populations. Therefore,
determining the functional contribution of each individual
cell in such a complex matrix requires the granularity of
a true single-cell interrogation methodology. The WT-Ovation
One-Direct System is a breakthrough for researchers developing
more effective diagnostic tools and targeted drug therapies
in immunology, neurobiology, hematopoiesis, and cancer stem
cell biology. We will present array data generated using
this amplification technology on a number test systems and
cell types. |
Greiner Bio-One Goes Green!
Greiner has added several environmentally friendly
features to their products and packaging. Click
here to read more about their green initiatives.
Greiner Bio-One produces high quality labware
such as tissue culture labware, microplates, serological pipettes
and tubes.
Labnet
- MultiGene Thermal Cyclers and Hermle Centrifuges are Now Available
from MJSBioLynx Inc.
We are pleased to announce that the
following products are now available from MJSBioLynx
Inc:
-
MultiGene Gradient Thermal Cycler - Produces consistent,
reliable results while making gradient technology affordable.
This unit is extremely simple to program, compact in design and
built to perform.
- MultiGene II Personal Thermal
Cycler - Combines a versatile and precise cycling unit
with easy to use software. Not limited to the amplification of nucleic
acids, the unit is also useful for applications such as enzymatic
digestion, ligation, and other procedures that require incubation
of small samples in a temperature controlled environment.
-
Hermle Centrifuges - We are pleased to re-introduce the
Hermle Line of Centrifuges. This product line offers a wide variety
of centrifuges, rotors and accessories to meet the needs of your
particular applications.
Labnet offers quality products for centrifugation,
liquid handling, as well as shakers and rockers, constant temperature
equipment, molecular biology equipment and laboratory plastics.
Please contact our Technical
Support Team for more information on these products or to
request a Labnet catalogue.
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