e-Lynx - March 2008Dear Researcher, We are pleased to present the March e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.
New SuppliersStressmarq
Biosciences Inc.
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| Product Number | Description | Pkg Size |
| NU430012 | One Colour Cy™3 ULS™ Labeling | 12 rxns |
| NU431012 | Two Colout Cy™3 & Cy™5 ULS™ Labeling | 12 rxns |
| NU340012 | WT-Ovation™ FFPE System | 12 rxns |
| NU340060 | WT-Ovation™ FFPE System | 60 rxns |
| NU330012 | WT-Ovation™ Pico System | 12 rxns |
| NU330060 | WT-Ovation™ Pico System | 60 rxns |
| NU310012 | Ovation™ RNA Amplification System V2 | 12 rxns |
| NU310060 | Ovation™ RNA Amplification System V2 | 60 rxns |
Trevigen
- Cultrex® Endothelial Cell Invasion Kit
Measure early angiogenesis
in vitro
The
Endothelial Cell Invasion kit was created in an effort
to accelerate the
screening process for compounds that influence vascular
endothelial 
cell
digestion
through and migration across basement extracellular membrane
(BME). This kit offers a
flexible, standardized,
high-throughput format for quantitating the
degree to which endothelial cells penetrate a BME barrier,
in vitro, in response to chemoattractants and/or
inhibiting compounds. This assay employs a simplified
Boyden chamber-like design with an 8 micron polyethylene
terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane],
a naturally occurring cancer chemopreventive agent, is
provided as a control for inhibition of in vitro endothelial
cell migration/invasion on Cultrex® Basment Membrane
Extract. Detection of cell invasion is quantified using
Calcein-AM. Calcein AM is internalized by the cells, and
intracellular esterases cleave the acetomethylester (AM)
moiety. Free Calcein fluoresces brightly, and this fluorescence
may be used to quantitate the number of cells that have
invaded or migrated using a standard curve.
| Product Number | Description | Pkg Size |
| TV3471096K | Cultrex ® Endothelial Cell Invasion Kit | 96 tests |
Greiner
Bio-One - ThinCert™ Plates
Cell culture plates for
tissue reconstruction in vitro
The novel
ThinCert™ plates offer an innovative solution for
air-lift culture with ThinCert™ cell
culture inserts. The deep wells of this plate allow the
application
of
a
larger medium volume during
the air-lift culture.
As a consequence, fewer medium changes are required and
the amount of manual work is significantly reduced. The
new ThinCert™ plates
incorporate notches for fixing the position of the inserted
ThinCert™ cell culture insert. Thus, the inserts
are guaranteed to
remain in a stable position during the entire cell culture
process.
| Product Number | Description | Pkg Size |
| GR657110 | ThinCert™ Plate, 6 well, sterile, with lid | 1 |
| GR665110 | ThinCert™ Plate, 12 well, sterile, with lid | 1 |
Greiner
Bio-One - Storage Plates for Acoustic Liquid Handling
Acoustic liquid handling is becoming an increasingly accepted technology for the accurate transfer of sub-microliter volumes within the HTS sector. With acoustic dispensing, ultrasonic pulses are used to accurately and precisely transfer solutions from a storage plate directly into the corresponding wells of an assay plate without contact, minimizing the risk of cross contamination. Greiner offers a comprehensive range of different storage plates that are specifically manufactured for acoustic dispensing. These plates are available in Cycloolefin (COC) and Polypropylene (PP)
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Standardized plate base thickness and density
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DMSO resistant PP and COC
Product Number Description Pkg Size GR781201906 384 well microplate, PP, F-bottom, natural 100 GR788860906 384 well microplate, COC, Small Volume, LoBase, Clear 80 GR783860906 1536 well microplate, COC, LoBase, clear 40 GR792870906 1536 well microplate, COC, Low Evaporation, clear 60 GR792891 Lid, for GR792870906, COC, clear, non sterile 120
Greiner Bio-One -
Non Binding Microplates
Solid bottom microplates with
an ultra-low binding surface
Greiner Bio-One introduces a new surface chemistry which is characterized by low protein, DNA and peptide binding properties to significantly imporve data quality, espcially in homogenous methods such as Fluorescence Polarization Assays (FPA) and Scintillation Proximity Assays (SPA).
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Low non specific biomolecule binding
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Non binding surface is stable under common assay conditions and does not degrade during short term storage
| Product Number | Description | Pkg Size |
| GR655900 | 96 well, solid bottom, black, non binding surface | 40 |
| GR781900 | 384 well, solid bottom, black, non binding surface | 40 |
| GR784900 | 384 well, solid bottom, Small Volume™, black, non binding surface | 40 |
Clear/White plates are available
on request.
White/Black µClear® plates are available on request.
Greiner
Bio-One - 384 Deep Well Small Volume Polypropylene Microplates
Microplates for reduced sample
consumption and assay miniaturization
In cooperation with the compound management group from Boehringer Ingelheim (Biberach, Germany), a novel well geometry was developed to enable perfect positioning for even small sample volumes and facilitate the direct transfer from storage to assay plate.
An optimal well design to directly address small sample volumes was determined in practical trials. A flat well bottom allows precise pipette tip positioning with aspiration close to the well bottom. With a standard microplate height (ANSI/SBS 2-2004) and 0.5 mm sealing rim, common plate handling processes such as heat sealing, piercing, stacking and transportation are easily employed without laborious robotic adaptation. A well geometry combination consisting of a square-top, conically tapered to a flat-bottom, renders the ideal design to utilize a high working volume with minimal waste of sample material.
| Product Number | Description | Pkg Size |
| GR784201 | 384 Deep Well Small Volume Polypropylene Microplate | 100 |
Micronic
- Rack Thawing Station
Defrost tube racks in less than 20 minutes
-
Fast Performance - defrost 96 tubes in less than 20 minutes, no warm-up time
- Small Footprint - uses little bench top space
- Specifications:
- Dimensions: 6.25'' W x 4.25''D x 11''H
- Weight: 5 lbs
- Electrical: 110-220 Vac 50/60 Hz
| Product Number | Description | Pkg Size |
| MSMP5140 | Rack Thawing Station | Each |
Assay
Designs - Hsp70 High Sensitivity ELISA Kit
For serum and plasma
Inducible heat shock protein 70 (Hsp70, or Hsp72) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. Hsp70 is essential for cellular recovery, survival and maintenance of normal cellular function. As a molecular chaperone, Hsp70 prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens and disease. Hsp70 is also released into the circulation from both necrotic and non-necrotic cells, and its levels in serum are increased by a number of stressors. Hsp70 is believed to serve as an immunological "danger signal," and measurement of Hsp70 in serum and plasma is a useful tool in the study of heat stress, infection, cardiovascular disease, exercise, and psychological stress.
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Ultra-sensitive ELISA for the measurement of Hsp70 in serum and EDTA plasma samples
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Fast results in just 4.5 hours
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Highly specific for inducible Hsp70 (Hsp72)
| Product Number | Description | Pkg Size |
| ADIEKS715 | Hsp70 High Sensitivity ELISA Kit | 1 x 96 wells |
G-Biosciences
- PEROXsay™ Quantitative Peroxide Assay
A modified ferrous oxidation-xylenol orange
(FOX) assay
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROXsay™ assay allows the ferric (Fe3+) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration. The assay has a Linear Range: 0-50µM
PEROXsay™ is suitable for the following applications; quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.
-
The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.
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PEROXsay-LIPID™ permits the quantification of peroxides in the presence of lipids without the need for extraction steps.
| Product Number | Description | Pkg Size |
| GB786440 | PEROXsay™ Quantitative Peroxide Assay | 250 Assays |
| GB786441 | PEROXsay-LIPID™ Quantitative Peroxide Assay | 250 Assays |
New Literature
G-Biosciences
2008/09 Catalogue
The new G-Biosciences catalogue features quality products for proteomics research, including protein gel stains, electrophoresis and Western blotting reagents, protein assays and protease inhibitors. They also offer an extensive line of educational products for hands on biotechnology teaching labs as well as kits for molecular biology and bioassays.
Assay
Designs - Hormones and Steroids Brochure
Assay Designs is the world leader in non-radioactive
hormone enzyme immunoassay kits
with an extensive product offering in steroid hormones,
peptide hormones, eicosanoids, and receptors.
Active
Motif - Epigenetics Brochure
Active Motif's new Epigenetics Brochure features antibodies and assay systems for chromatin biology and transcription. These products include DNA methylation assays, chromatin assays and IP kits, transcription factor assays, as well as related antibodies.
Promotions
Macherey-Nagel
- iPod® Promotion
Until June 30, 2008
Take advantage of special pricing on
NucleoSpin® Kit Bundles
AND receive a FREE iPod nano
Click
here for details
Offer valid from March 1st to
June 30th, 2008
Cannot be combined with any other offers or
discounts
Promotional Code MCNI08
Abgent - 40% OFF Newly Released Antibodies
Abgent strives to provide you with an expansive and unique antibody collection for advancing your research.
Abgent now offers huge savings
on new products.
Click here for
a listing of applicable products and promotion details
Assay
Designs - Buy 3, Get 1 Free - Antibody and Protein Promotion
Offer extended until March 31st, 2008
Fall into savings with this buy 3, get 1 free promotion on Assay Designs top selling antibodies and proteins. All you need to do is select at least 4 Assay Designs™ antibodies or proteins from the list below and you will receive the lowest priced product free of charge.
-
Over 110 antibodies and proteins to choose from, including many Stressgen® brand products
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Diverse set of research areas including heat shock, cell signaling, oxidative stress, and inflammation
-
Superb performance documented in thousands of peer-reviewed publications
Click here for promotion details
Valid until March 31st, 2008
or while quantities last
Promotional Code: EOY112607
Cannot be combined with any other offers or discounts
Technical Report
miRtect-IT™:
A Novel Method for Small RNA Detection
Tech Tip by USB Corporation
The recent discovery and characterization
of small non–protein–coding regulatory RNAs,
such as microRNAs (miRNAs), PIWI–associated RNAs (piRNAs
and rasiRNAs), short interfering RNAs (siRNAs), trans–acting
siRNAs (ta–siRNAs), and other families of short RNAs
has led to a rapid expansion of research directed at elucidating
their expression patterns and regulatory functions(1,2).
Currently, Northern blotting is the standard method for
the detection of small RNAs, because it allows direct comparison
of the quantity of small RNA between different samples.
However, major drawbacks of Northern blotting are time consuming
procedures and poor sensitivity, especially when monitoring
expression of short nucleotide sequences. Despite the
improvements in detection sensitivity provided by Locked
Nucleic Acid (LNA) substituted probes(3), Northern blotting
requires relatively large amounts of starting material and
involves multiple handling steps (Table 1).
Another approach for small
RNA detection is based on the ribonuclease protection assay
(RPA), which takes advantage of liquid hybridization kinetics
to improve detection sensitivity (Table 1). Both Northern
blotting and RPA determine the amount of small RNA by measuring
the amount of the labeled probes that are non–covalently
hybridized to the small RNA, providing a relative quantification
of small RNA levels. In addition, the critical experimental
points that determine the assay efficiency, which are the
hybridization and wash steps in the Northern Blot assay
and the hybridization and ribonuclease treatment in RPA,
usually require optimization because insufficient
treatment may lead to non-specific detection while excess
treatment may compromise the detection sensitivity.
USB offers a novel approach for direct labeling and detection of small RNAs. The miRtect-IT™ technique takes advantage of liquid hybridization kinetics to improve detection sensitivity, but without a procedure that requires optimization. Similar to Northern blotting, miRtect-IT™ does not require specialized equipment or any amplification step, and thus allows direct and accurate measurement of specific small RNAs.
Click here to read the full article
Tradeshows
Visit MJSBioLynx at the following events:
| Tradeshow | Location | Date |
| Southern Alberta Scientific Equipment Tradeshow | University of Calgary | March 12, 2008 |
