e-Lynx - March 2009Dear Researcher, We are pleased to present the March e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.
|
Product
Number |
Description |
Pkg
Size |
| TV3490096K | CultreCoat® BME 96 Well Cell Adhesion Assay | 96 wells |
| TV3491096K | CultreCoat® Laminin I 96 Well Cell Adhesion Assay | 96 wells |
| TV3492096K | CultreCoat® Collagen I 96 Well Cell Adhesion Assay | 96 wells |
| TV3493096K | CultreCoat® Collagen IV 96 Well Cell Adhesion Assay | 96 wells |
| TV3494096K | CultreCoat® Fibronectin 96 Well Cell Adhesion Assay | 96 wells |
| TV3495096K | CultreCoat® Vitronectin 96 Well Cell Adhesion Assay | 96 wells |
| TV3490696K | CultreCoat® BME 96 Well Adhesion Protein
Array Assess adhesion on Laminin, Collagen I, Collagen IV, Fibronectin, and Vitronectin |
96 wells |
Please contact our Technical Support Team for more information about these products.
Meridian
Life Sciences - Tru Block™
Heterophilic Blocker
Put the Brakes on False Positive Results with the
Most Powerful Heterophilic Blocker
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Effective against Heterophilic Antibodies to mouse (HAMA) and other animals
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Extensive Quality Control
-
HAMA Assay run on each lot
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Large lot capability
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Lot-to-lot consistency
Product
Number |
Description |
Package
Size |
| BDA66800H | TRU Block™ | 100 mg |
Please contact our Technical Support Team for more information about this product.
New Literature
Greiner
Bio-One – Labware Product Brochure
This new brochure features Greiner
Bio-One’s quality labware for cell culture as well as their
light protection tubes, shorty pipettes, half-area microplates
and much more.
Click here
to view the PDF version.
Active
Motif – Motifvations Newsletter – March 2009
This issue features an article on Retroviruses, Chromatin Structure and Transcriptional Regulation. It includes information on magnetic chromatin immunoprecipitation, chromatin assembly, methylated DNA analysis and histone purification.
Assay
Designs – 2009-10 Catalogue
The new catalogue contains information on Assay Designs products, including multiplex kits, immunoassay kits, activity/detection kits, antibodies and proteins. They are specialized in the fields of Heat Shock, Apoptosis, Cell Signaling, GPCR, Neuroscience, Oxidative Stress, etc.
Chromatographic
Specialties Inc - Chromspec Chronicle
- YMC - Column and C18 selection guides
- Agilent Bio-Monolith HPLC Columns - easing your separation anxieties.
- CHROMSPEC™ lamps for Waters® Detectors
- New Supplier: Vortex - dissolution solutions.
- Looking for hard to replace parts for your GC or LC Instrumentation? CSI has the answer.
Click here to view the online PDF.
Contact our Technical Support Team or call 1-888-593-5969 to request a your copies!
Promotions
Polyplus Transfection - Special
Introductory Pricing on jetPRIME™ DNA Transfection Reagent
-
Outstanding efficiency with extremely low toxicity
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Uses low amounts of DNA - Uses only 2 µg of DNA per well in 6-well plates
- Efficient transfection into mammalian cell lines such as HeLa, HEK-293 and CHO cells
-
Very Convenient: No medium changes required, compatible with serum and antibiotics
For a limited time, take
advantage of introductory prices.
Click here
for pricing details.
Offer valid until April 30th, 2009
Samples are available - contact our Technical Support Team
-
Animal
Product Free Media Optimization Kit -
FNC Coating Mix® - complete serum-free medium; increases attachment of cells
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Atholate™ Packets - proprietary blend of plant protein hydrolysates that are APF Certified™ and match the performance and nutritional characteristics of standard casein hydrolysate
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The first 10 customers who order AthenaES® products from MJSBioLynx will receive a free AthenaES® T-Shirt.
Click here for promotion details
Offer valid until March 31, 2009
or while quantities last
Cannot be combined with any other offers or discounts
Greiner Bio-One - Free Pizza Party Promotion is Back!
For the cold Canadian winter months
of January to March 2009 - enjoy hot, delicious pizza on us!
Our thank you to our wonderful MJSBioLynx customers.
Accumulate $500 in Purchases of Greiner Bio-One Products from MJSBioLynx and Receive a $50 Pizza Gift Certificate.
Click
here for more information.
Greiner Bio-One offers high quality labware such as cell culture labware, microplates and tubes.
Limit 2 Pizza Parties per Lab
Cannot be combined with other offers
Please refer to promotional code GR1309
Assay Designs - 50% OFF an ImmunoSet Buffer Pack with the Purchase of Any ImmunoSet
The
Assay Designs ImmunoSet Buffer Pack provides an all-purpose
buffer that is easily modified to coat, block, and wash plates
in addition to diluting standards and samples. TMB substrate
and acid stop solution for colour development and reaction termination
are also supplied.
This offer is valid until April 30th, 2009
Click here for details.
Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand
ChIP-IT™
Express HT allows you to perform chromatin IP in a fast, reproducible
high-throughput format. It combines the efficiency of the groundbreaking
magnetic bead-based ChIP-IT™ Express Kit with a 96-well
plate format, enabling the rapid and efficient processing of
a large number of ChIP reactions.
This offer is available while quantities last
Click here for promotion details
Technical Report
The DUALmembrane System – Detection of Novel Interaction Partners for Integral Membrane Proteins - by Dualsystems Biotech
Protein-protein interactions play a crucial role in every living organism and the information about protein(s) associated with a certain protein of interest (so called “interactors”) largely contributes to our understanding of cellular processes at the molecular level. A wide range of fundamental biological processes such as cell signaling, transport of membrane-impermeable molecules, cell-cell communication, and cell adhesion are mediated by membrane proteins. Due to their pivotal role in many cellular processes, their direct link to human diseases and their extracellular accessibility to drugs, the identification of proteins associated with integral membrane proteins is desirable. No wonder that, for the above-mentioned reasons, there is a strong demand from both academic researchers and biotech-pharma companies to gain further insight into pathways and interactions involving membrane proteins. However, due to their complex chemical properties, membrane proteins are very hard to manipulate, making the study of their corresponding interactors even more challenging.
The
DUALmembrane System reliably detects interactions between integral
membrane proteins and receptor subunits and co-receptors, as well
as membrane-associated proteins and soluble proteins. The system
is based on the properties of ubiquitin, a small protein modifier
that is conserved in all eukaryotes and plays a primary role in
protein degradation. In this system, the reporter protein is a
fusion of the DNA-binding domain of the LexA protein and the Herpes
simplex VP16 transactivator. The reporter is fused to the Cub
moiety, which in turn is fused to an integral membrane protein
(depicted in red, called Cub-TF). A transmembrane prey protein
(depicted in green) is fused to the NubG moiety. The only requirement
is that both Cub and NubG are located on the cytoplasmic face
of the membrane. Co-expression of Bait-Cub-TF with a non-interacting
Prey-NubG does not lead to the formation of split-ubiquitin nor
cleavage by UBPs, resulting in cells that are His3- and lacZ-
(left panel). If bait and prey interact, Cub and NubG are brought
into close proximity, where they will form split-ubiqutin, resulting
in cleavage and liberation of the TF reporter. The reporter is
now free to enter the nucleus, where it will bind and activate
the reporter genes to result in cells that are His+ and that turn
blue in a ß-galactosidase assay.
DUALmembrane
baits
In the DUALmembrane system, a given transmembrane bait can either be a Type I (C-terminus in the cytosol) or Type II (C-terminus outside the cell or in the lumen of ER, Golgi etc.) transmembrane (TM) protein. In the case of a Type I TM protein, the Cub-TF portion is fused to its C-terminus so that the Cub-TF portion faces the cytosol, thus generating a Bait-Cub-TF fusion. If a bait to be studied in the DUALmembrane system is a Type II TM protein, the TF-Cub portion has to be fused to N-terminus (TF-Cub-Bait). In both cases, an interaction between an integral membrane protein and a prey protein will result in the cleavage after the last amino acid of the Cub domain, thus liberating either TF (in the case of the Type I TM bait) or TF-Cub (in the case of Type II TM bait).
DUALmembranes
preys
In the Dual membrane system, a given prey protein (or a cDNA library) can be studied in either Y-NubG (where Y is cDNA or genomic DNA insert) or NubG-Y orientation. In the case of a Type I TM prey, the cDNA (or a library of cDNAs encoding potential interactors) is fused at its C-terminus with the NubG domain, whereas in the case of a Type II prey, it is fused at its N-terminus with the NubG domain. In this way, it is possible to identify both Type I (Y-NubG orientation) and Type II (NubG-Y orientation) transmembrane proteins that interact with a particular membrane bait protein.
The DUALmembrane System is available from Dualsystems Biotech in both convenient kit form and as a custom screening service. The DUALmembrane starter kits screen full-length integral membrane proteins for interactions in vivo under the physiological conditions at the membrane, with novel interactions identified through screening of cDNA libraries. It also allows easy subcloning of full-length cDNA inserts using Sfi I restriction site. With the custom DUALmembrane screening service, researchers can save time and money by leveraging Dualsystems Biotech’s extensive experience and expertise in identifying novel interaction partners for proteins of interest. Custom screening is particularly useful for detecting weak or transient interactions.
Dualsystems Biotech is focused on the development of products and services in the emerging area of interactive proteomics. Their areas of expertise include genetic screening in yeast, screening for protein-protein interactions, yeast expression systems and construction of cDNA libraries. Please contact the MJSBioLynx Inc. Technical Support Team for more information on products from Dualsystems Biotech.
Tradeshows
Visit MJSBioLynx at the following tradeshows.
Tradeshow |
Location |
Date |
| Southern Alberta Scientific
Equipment Tradeshow |
University of Calgary | March 11, 2009 |
Announcement
Macherey-Nagel - KitFinder Tool for Nucleic Acid and Protein Purification
Macherey-Nagel
introduces a new feature of their website, the Bioanalysis KitFinder
by Application. This new tool guides you to recommended Macherey-Nagel
when you enter information about your application, sample material
and daily throughput.Once the search is
complete, you will obtain the specification and application data
for the kit that best suits your needs.
Visit www.mn-net.com/BioKitFinder to start using this tool.
Please contact our Technical Support Team for more information on purification products from Macherey-Nagel
