• Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready-to-use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Complete kit, includes: NucleoSpin Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion and Protein Solving Buffer for solving all types of proteins
  

• Flexible Evaluation - Use anywhere from 4 to 96 test wells per experiment; it is your choice.
• More Data Per Well- Analyze cells using multiple fluorescent probes, labels or stains by using a microplate reader, microscope or high content imaging system.
• Creative Assay Design- Coat any ECM or BME on the plate to create a 2-D or 3-D environment for cell migration or cell invasion assays.
• Reproducible Results- Achieve lower well-to-well CV's with the unique Oris™ assay design than with scratch assays.
• Real-time Monitoring- Track changes in cell movement and morphology as cell migration or invasion progresses.
• HTS and HCS Compatibility- Observe cells directly without interference from cell culture inserts or transmembrane devices.

• Fast magnetic protocol completed in less than 3 hours
• Optimized reagents and protocol ensure successful capture from as few as one unmethylated CpG dinucleotide per fragment
• Provides DNA suitable for use in many downstream applications, such as real time or endpoint PCR analysis of the methylation status of particular loci, sequencing, or amplification and labeling for microarray analysis.

Click here to view the UnMethylCollector™ manual.

Active Motif - MethylCollector™ Ultra
Methylation analysis from as few as 200 cells

MethylCollector™ Ultra* is an improved technology for the enrichment of CpG-methylated DNA. The kit combines the His-tagged MBD2b protein with its binding partner MBD3L1 to generate higher affinity and better specificity for CpG-methylated DNA than other methyl-binding protein or antibody immunoprecipitation methods.

• High affinity binding provides greater enrichment than other MBD capture or antibody-based immunoprecipitation methods
• Specifically detects methylated CpGs from 1 ng - 1 µg of DNA fragmented by sonication or enzymatic digestion
• No need to denature DNA; simple protocol completed in less than 3 hours

Click here to view the MethylCollector™ Ultra manual

* Technology is covered under U.S. Patent No. 7.425.415

Labnet - Revolver 360° Sample Mixer

Labnet's Revolver accepts a variety of tube sizes and mixes them horizontally, vertically, or any position in between. Changing the mixing angle is easy! simply turn the rotisserie. No tools are required.

• Complete 360° rotation
• Adjustable 0-90° mixing angle
• Interchangeable rotisserie options for all common lab tubes.
• Independently adjustable rotisseries
• Cold room and incubator compatible
  

Simport - EconoTube™

• 1.5 mL tube, made of Polypropylene
• Economically priced
• Specially designed to reduce popping during centrifugation, freezing, storing or shipping
• Reinforced bottom for added protection against leakage
• Can be used for centrifugation up to 20,000g

For pricing information, contact our Customer Service Team at 1-888-593-5969 or sales@biolynx.ca.

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New Literature

Active Motif - Transcription Biology Brochure

This new brochure features transcription factor and nuclear receptor ELISAs, chromatin assays and IP kits, luciferase assays, supershift & gelshift assays.
Click here for the on-line PDF.
Contact our Technical Support Team to request printed copies.

Associates of Cape Cod Inc. - LAL Update, April 2009

In this issue: Controls for Photometric Tests: Implications for Low Capacity Test Systems
by Robert Porzio and Michael Dawson, Ph.D., RAC.
Click here for the on-line PDF.

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Promotions

Meridian Life Sciences - Free Pilot Runs for Antibody Production

Meridian Life Science, Inc. (MLS) is offering a limited number of Free Pilot Runs of ascites production of monoclonal antibodies. This time sensitive promotion is for qualified companies who have needs for bulk production.

• Small pilot lots are offered to optimize production yields
• Significant cost saving over cell culture method or even in-house production methods
• Lot-to-lot yield and quality is highly consistent
• Antibody concentration in ascites is determined on each lot by electrophoresis
• Quick turnaround is achieved by maintaining a ready supply of BALB/c and CAF1 mouse colonies
• Purification can be performed to customer specifications

Please contact our Technical Support Team to inquire.

Abgent - Free Chocolate with the Purchase of Antibodies

From April 1st to June 30th, 2009
Accumulate purchases of 5 Abgent antibodies* and receive a $50 gift card for Laura Secord Chocolates.

Abgent antibodies are widely used in the fields of :

Click here for details.
* Offer is not applicable to Abgent Antibody Sampler Kits.

Gentel Biotechnologies - Receive a Free SIMplex™ 16 Multi-Array System when you purchase an APiX™ Cancer Biomarker Array Kit.

Measure the relative abundance of 46 human cancer-related proteins
with the single-capture antibody array.

• Reliable profiling data of 46 cancer proteins per serum sample
• A complete kit with a pre-printed array and labeling reagents
• Compatibility with standard microarray scanners

Use the arrays with the SIMplex™ 16 Multi-Array System for:

• Leak-proof microarray separation
• Increase throughput to process 16 samples simultaneously
• Compatibility with multi-channel pipettors

Click here for promotion details.
Offer valid from April 1st to May 29th, 2009

Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand

ChIP-IT™ Express HT allows you to perform chromatin IP in a fast, reproducible high-throughput format. It combines the efficiency of the groundbreaking magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate format, enabling the rapid and efficient processing of a large number of ChIP reactions.

This offer is available while quantities last
Click here for promotion details

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Technical Report

Heat Shock Protein 90 (Hsp90)
by Dr. Ariel Lowrier, StressMarq Biosciences Inc.

Hsp90 is one of a number of classes of heat shock proteins (Hsps), which are generally synthesized in response to cellular stress as well as being constitutively expressed in some cases. These types of stress are numerous, but include heat shock, metal toxicity, nutrient deprivation, oxidative stress as well as numerous disease states of which cancer has received the most attention. Hsps function is several ways, but have the ability to form complexes with client proteins and act as molecular chaperones. These complexes are themselves directly involved in protein folding in the cytosol, endoplasmic reticulum (ER) and mitochondria, the intracellular transport of proteins, protein repair and degradation (of environmentally damaged proteins), regulatory protein control, and the re-folding of misfolded proteins. The Hsps have been generally classified into several classes depending on their molecular weights, see Table 1.

*Adapted from Annals in Oncology, 14: 1169-1176, 2003

Hsp90 is one of the most prevalent chaperone proteins present in the cell – it can represent 1-2% of unstressed cell’s protein and as much as 6% in the stressed cell. It has the ability to provide multi-component complexes which have been shown to include p60/Hop, p50Cdc37, Hsp40/HDJ2, p23, Hsp70 and one of a number of immunophilins (such as FKBP51 and FKBP52). Hsp90 itself is present in two forms, Hsp90a and ß. Both proteins are highly related and appear to have identical activities, however, the former is inducible whereas the latter is constitutively expressed. Hsp90 is also a phospho-protein – and has two or three bound phosphate molecules per monomer and exists as a homodimer. One of the clear distinctions that have made it a potential drug target are the fact that a significant amount of its client proteins are protein kinases. These client proteins include ErbB2/Her-2, EGFR, Hif1a, c-Met, Akt/PKB, Raf-1, Cdk-1 and 4, Aurora B, Ask1, CHK1, CKII as well as mutant p53. Furthermore, due to the nature of the interactive and complex network that is associated with regulating cell proliferation and cell death, the approach of identifying one specific (drug) target and blocking or activating it can result in the cell simply altering it’s regulation in order to maintain the phenotype. The wide amount of Hsp90 client proteins and the fact that many of them are directly involved in regulatory (signaling and phosphorylation) processes have allowed researchers to take a wider target approach to shutting down activities associated with cancers and stepping around the redundancies of the multiple signaling routes.

Hsp90 has been widely studied in relation to steroid receptors, despite the first recognized substrate being discovered to be pp60v-sr. These studies have elucidated that the receptors must be bound to Hsp90 to have a high binding affinity for steroids. The Hsp90 assembly also showed the importance of the presence of p23 and Hop, working together with Hsp70 and Hsp90 as a multi-protein machine. Together with Hsp40, these five proteins work together to produce receptor-Hsp90 heterocomplexes with steroid-binding activity. Mechanistic studies have indicated the steps involved in the assembly, starting with two sequential ATP-dependent events involving first hsp70 and then hsp90. Interestingly, since the chaperone machinery reveals a hydrophobic steroid-binding cleft to access the ligand, the action of Hsp90 as part of the multi-protein machinery is quite different from that of hsp90 acting alone in vitro to promote reactivation of partially denatured enzymes. The three hsp90-binding tetratricopeptide repeat (TPR) domain immunophilins - FKBP52, FKBP51, and CyP-40- were discovered as components of steroid receptor-hsp90 heterocomplexes.

Steroid-mediated glucocorticoid receptor translocation from the cytoplasm to the nucleus has led to the discovery that the TPR domain immunophilins link Hsp90 to a dynein motor protein. The mechanistic conclusions derived from elucidation of how the Hsp90 machinery works with the steroid receptor appears to provide a broad set of principles of how Hsp90 works, which would be in contrast to other cell stress protein chaperone studies. The latter would indicate that in order to be a substrate for Hsp90 or Hsp70 the presence (some degree) of unfolded protein would be required – however there have been no indications for the steroid receptor studies that receptors are in any way denatured. When Hsp90 and Hsp70 act together a highly focal site of attack is located on the glucocorticoid receptor that is situated on the surface of the ligand-binding domain at the opening of the hydrophobic steroid-binding cleft. However, whether or not Hsp90 has the general ability to recognize cleft openings on other signaling proteins is unclear, but due to the fact that they are present on almost all protein as a topological feature it is possible that the Hsp90 and Hsp70 complex recognizes these native rather than denatured conformations.

Currently, high interest has been manifest in benzoquinone ansamycin antibiotics (BA), which include herbimycin, geldanamycin and 17-allylamino-17-demethoxygeldanamycin (17-AAG), a drug class capable of affecting multiple targets in tumor cell pathways by binding to Hsp90. 17-AAG has also been modified to make 17- dimethylaminoethylamino - 7 - demethoxygeldanamycin (17-DMAG) to improve drug half-life and water solubility. These drugs bind Hsp90 at a conserved 25kD N-terminal domain which is responsible for ATP binding, hence disrupting the Hsp90 chaperone complex. Other inhibitors include radicicol, the chemotherapeutic agent cisplatin, taxol, as well as the antibactericide novobiocin. Cisplatin binds to a novel nucleotide-binding site of Hsp90 at its C-terminus, while novobiocin binds to several domains of Hsp90. This leads directly to the disruption of the Hsp90 activity and the chaperone complex functions, resulting in depletion of the oncogenic client proteins.

StressMarq Biosciences Inc has endeavored to provide a full series of unparalleled hsp90 reagents to allow further study of the Hsp90 chaperone system in vivo and in vitro. Although not exhaustive, the following table shows the unparalleled position StressMarq has created amongst suppliers.

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Events

Tradeshows

Meet ^MJSBioLynx at the following events:

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