e-Lynx - November 2008Dear Researcher, We are pleased to present the November e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.
New SuppliersAthenaES™ - Simple Solutions for Complex Proteins
AthenaES™ is a biotechnology supplier of research reagents for pharmaceutical and industrial applications. They have an extensive line of products including protein expression products, cell culture products, protein refolding reagents, specialty proteins, and enzyme assays. All of their products are made with attention to quality, reliability, and usability. Visit www.athenaes.com for more information
Biosensing Instrument - Surface Plasmon Resonance Instruments
Biosensing Instrument Incorporated is devoted to providing high-performance Surface Plasmon Resonance (SPR) instruments for creative research and efficient analysis. Using innovative designs, their instruments offer high sensitivity for detecting analytes of various sizes, a wide time response range for slow and fast kinetics, and maximum versatility and flexibility. Applications Include:
Trevigen - HT Chemiluminescent
PAR Sandwich ELISA
|
| Product Number | Description | Pkg Size |
| MSMP35015 | Electric Capper | Each |
Featured Product
Trevigen - Cultrex®
Cell Invasion Assay Kits
Trevigen’s Cultrex® Cell Invasion Assays were originally created in an effort to accelerate the screening process for compounds that influence cellular invasion through extracellular matrices, which is fundamental to angiogenesis, embryonic development, immune responses, and tumor cell metastasis.
| Product Number | Description | Pkg Size |
| TV3455096K | Cultrex® BME Cell Invasion Kit | 96 Wells |
| TV3455024K | Cultrex® BME Cell Invasion Kit | 24 Wells |
| TV2456096K | Cultrex® Collagen I Cell Invasion Kit | 96 Wells |
| TV2456024K | Cultrex® Collagen I Cell Invasion Kit | 24 Wells |
| TV3457096K | Cultrex® Collagen IV Cell Invasion Kit | 96 Wells |
| TV3457024K | Cultrex® Collagen IV Cell Invasion Kit | 24 Wells |
| TV3458096K | Cultrex® Laminin I Cell Invasion Kit | 96 Wells |
| TV3458024K | Cultrex® Laminin I Cell Invasion Kit | 24 Wells |
| TV3460024K | Cultrex® CultreCoat® BME Cell Invasion Kit | 24 Wells |
Please contact our Technical Support Department for more information
New Literature
Associates
of Cape Cod, Inc. - 2008 Catalogue
This new catalogue provides information on a full range of products for endotoxin and glucan detection and quantification.
Click here to request a copy!
TubeWriter
is an automated, direct-print system for fast,
easy-to-read, permanent sample labeling. -
Prints on ANY tube, microscope slides, and plates
-
Prints on the side OR cap of tube
-
No consumables or ink cartridges: Uses the pens you already have
-
No adhesives or labels to apply
-
No handwriting necessary!
-
Simple Excel input: type label data and click "PRINT"
-
TubeWriter can label up to 20 tubes in 5 minutes
This offer is available until December 31st, 2008
Click here for additional information
Platypus
Technologies - Free Poster/Calendar Offer
Purchase any Oris™ Cell Migration or Cell Invasion Kit and receive a FREE 2009 Platypus Poster/Calendar for your lab.
The Oris™ assays use an industry standard, 96-well microplate populated with unique Oris™ Cell Seeding Stoppers in each well. These patent pending, stoppers allow researchers to dispense cells into each well and create an exclusion zone with the tip of the stopper. After the cells adhere to the well surface, the stoppers are removed creating a 2 mm diameter circle into which the cells can then migrate. Researchers can then take real time photos (digital image) of the cells migrating into this central detection zone and quantify the results using microplate readers all in the same well.
This offer is available
until December 31st, 2008 or while quantities
last
Please reference promotional code PPPC08
Click here for additional information
Micronic - Incredible Savings on Products for Sample Storage and Traceability
Micronic
offers a unique product line that ensures sample
integrity and traceability. For a limited time,
take advantage of special pricing on selected
products including Traxis and Tracker starter
packs.
This offer is available
until November 30th, 2008
Cannot be combined with any other offers or discounts
Please reference promotional code MSSP08
Click here for promotion details.
USB
- Spend $150 on USB Biochemicals and
we'll send you the USB fitness pack Free!
USB
provides biochemicals that deliver reliable and
consistent results. USB biochemicals and Ultrapure
brand products meet the highest industry standards
for quality. They are available in a variety of
grades such as ACS Reagent Grade, CP Grade (Chemically
Pure), USP Grade (USBioAnalyzed) and Molecular
Biology Grade.
Don't forget about their line of convenience reagents. USB offers an extensive selection of Ultrapure biochemical reagents in premixed, ready-to-use formats for convenience in any lab.
Now's a great time to step forward and purchase USB Biochemicals and convenience reagents. When you spend $150 or more, we'll send you a USB water bottle and pedometer to track your own fitness results.
This offer is available
while quantities last
Please reference promotional code 28H-2300W
Click here for promotion details.
Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand
ChIP-IT™ Express HT allows you to perform chromatin IP in a fast, reproducible high-throughput format. It combines the efficiency of the groundbreaking magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate format, enabling the rapid and efficient processing of a large number of ChIP reactions.
This offer is available while quantities last
Click here for promotion details
Assay
Designs - Buy 3, Get 1 Free - Antibody and Protein
Promotion
Offer extended until December 31st, 2008
Fall into savings with this buy 3, get 1 free promotion on Assay Designs top selling antibodies and proteins. All you need to do is select at least 4 Assay Designs™ antibodies or proteins from the list below and you will receive the lowest priced product free of charge.
Click here for promotion details
Technical Report
High Resolution Analysis
of the Human Transcriptome: Detection of Extensive Alternative
Splicing Independent of Transcriptional Activity
By ExonHit Therapeutics
Abstract
Commercially available microarrays have been used in many settings to generate expression profiles for a variety of applications, including target selection for disease detection, classification, profiling for pharmacogenomic response to therapeutics, and potential disease staging. However, commercial microarrays do not resolve a large portion of the transcriptome, as most of these transcripts are produced by alternative splicing. The inconsistency between genes and transcripts as explained through alternative splicing, is a major mechanism for driving proteomic diversity through transcript heterogeneity. Recent advances in the design of expression arrays and bioinformatics analysis techniques for the identification of alternative transcripts have produced a unique microarray design that allows the detection of alternatively spliced events within the human genome through the use of exon body and exon junction probes to provide a direct measure of each transcript, through simple calculations derived from expression data. Over 138,000 putative events were identified with direct evidence of publicly available cDNA sequences, with novel exons as the most abundant type of splice event detected. A custom array was manufactured to detect splicing events in the human genome and the performance was measured against standards recently published (MAQC Project) and the array monitored over 400,000 potential splicing events (known and predicted). The array was shown to be highly quantitative through sample titration for probe, gene and splice event level analysis. The array highly correlated with the Affymetrix HG-U133 Plus 2.0 array on the gene level, and provided more extensive coverage of each gene. Almost 60% of genes demonstrating differential expression of greater than 3 fold also contained extensive splicing alterations. Also, almost 10% of genes having constant overall expression values contained evidence of transcript diversity when examined in detail. Two examples illustrate the types of events identified: Lim domain 7 showed no differential expression, but demonstrated an exon skip event, while Erythrocyte membrane protein band 4.1 –like 3 was differentially expressed 11 fold and contained an skipped exon isoform. A novel design for the detection of specific alternatively spliced transcripts is described and shown to be highly reproducible, quantitative and provides high resolution for transcript analysis. Significant changes were also detected independent of transcriptional activity, indicating that the controls for transcript generation and transcription are distinct, and require novel tools in order to detect changes in specific transcript quantity. This array design will provide researchers with the ability to identify and quantify specific changes not only at the gene level, but also at the transcript level.
Click here to read the complete article
Events
Web Lecture: Linear cDNA
amplification for archiving, distributing and analyzing
large numbers of limiting clinical samples via QPCR
Date and Time:
Tuesday, November 18, 2008 8:30 am
Pacific Standard Time (GMT -08:00, San Francisco)
Duration: 1 hour 15 minutes
Click here to register.
Description: The utilization of QPCR for large scale, focused, gene expression studies is becoming a common application in the development and implementation of molecular diagnostic tools. To this end, large biorepositories are being asked to provide biologicals that are suitable for gene expression analyses. Given the lability of RNA and the inherent variability associated with reverse transcription, not to mention the limiting amounts of RNA extracted from precious clinical samples, there is a push to establish guidelines for the amplification and distribution of cDNA as the primary source of nucleic acid for expression analysis. The pre-amplification process driven by SPIA provides the utility and flexibility to perform a large number of downstream gene expression analyses all the while conferring lab to lab reproducibility at an unprecedented level. This seminar will discuss the workflows employed for pre-amplification of large RNA collections and demonstrate specific examples of QPCR gene expression analysis using linearly amplified cDNA across many sites from a single RNA source.
Presenter: Andrew
I. Brooks, PhD
Dr. Brooks is the Director
of the Bionomics Research and Technology Center
(BRTC) at the Environmental and Occupational Health
Science Institute of the University of Medicine
and Dentistry of New Jersey. He is also the Associate
Director of Technology Development at Rutgers University’s
Cell and DNA Repository and an Associate Professor
of Environmental Medicine and Genetics at UMDNJ.
Previously, Dr. Brooks was the Director of the Functional
Genomics Center at the University of Rochester Medical
Center. Dr. Brooks is a molecular neuroscientist
whose research interest in deciphering the molecular
mechanisms underlying memory and learning includes
the study of neurotoxicants and their effects on
development and cognitive decline associated with
disease onset and progression. In the past, Dr.
Brooks has served as the Director of the Academic
Medicine Development Company (AMDeC) Microarray
Resource Center (MRC) and as Chair of the Microarray
Research Group (MARG) in the Association of Biomedical
Resource Facilities (ABRF).
