e-Lynx - September 2008Dear Researcher, We are pleased to present the August e-Lynx, a newsletter devoted to keeping our customers up-to -date on new technologies, our new products, suppliers, activities and promotions.
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| Product Number | Description | Pkg Size |
| AM53018 | ChIP-IT™ Express HT | 96 rxns |
| AM90096 | MAG-96 Magnetic stand | Each |
Click here for Promotional Offer
Active
Motif - Recombinant Methylated Histones
Histones with Site- and Degree-specific Methylated
Lysines
Histones with specific methylation states are an essential tool for investigating which methylation patterns are key to complex functional questions about chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair.
| Product Number | Description | Pkg Size |
| AM31207 | Recombinant Histone H3 (C110A) | 50 µg |
| AM31208 | Recombinant Histone H3 monomethyl Lys4 | 50 µg |
| AM31209 | Recombinant Histone H3 dimethyl Lys4 | 50 µg |
| AM31210 | Recombinant Histone H3 trimethyl Lys4 | 50 µg |
| AM31211 | Recombinant Histone H3 monomethyl Lys9 | 50 µg |
| AM31212 | Recombinant Histone H3 dimethyl Lys9 | 50 µg |
| AM31213 | Recombinant Histone H3 trimethyl Lys9 | 50 µg |
| AM31214 | Recombinant Histone H3 monomethyl Lys27 | 50 µg |
| AM31215 | Recombinant Histone H3 dimethyl Lys27 | 50 µg |
| AM31216 | Recombinant Histone H3 trimethyl Lys27 | 50 µg |
Trevigen
- Cultrex® Laminin I (Mouse) PathClear®
Laminin is an extracellular matrix protein which contains a number of functional domains that allow it to assemble into sheets. Trevigen's mouse laminin is purified from EHS sarcoma.
The PathClear® designation means that in addition to standard sterility, endotoxin and MAP testing, the Laminin is tested by PCR and is clear of 31 pathogens and viruses, including LDEV. Each lot is rigorously qualified in biological performance assays.
| Product Number | Description | Pkg Size |
| TV340001002 | Cultrex® Laminin I (Mouse) PathClear® | 1mg |
Trevigen - Superoxide Dismutase Assay Kit Cuvette Format
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Assay is ideal for assaying SODs in mammalian tissue and cell lysates in a cuvette format
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The assay is performed in as little as 10 minutes
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Suitable for the assay of isozymes SOD1 (cytosolic Cu/Zn-SOD), SOD2 (mitochondrial Mn-SOD), and SOD3 (extracellular Cu/Zn-SOD)
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Applications: Calculating SOD activity by measuring the reduction of WST-1.
| Product Number | Description | Pkg Size |
| TV7502100K | Superoxide Dismutase Assay Kit - Cuvette Format | 100 tests |
Pickering Laboratories - Post-Column Derivatization
Our
sister company, Chromatographic Specialties Inc., now offers
products from Pickering Laboratories. Pioneers in post-column
derivatization technology and chemistry, Pickering Laboratories
have been developing applications to solve the analysis requirements
of government and private labs for over 20 years. They are leaders
in the field of HPLC and post-column derivatization technology,
having developed instruments and chemistry to solve analysis
challenges in the field of environmental and clinical testing.
Pickering Laboratories are currently focusing on the Clinical
and Pharmaceutical laboratory analyses of Amino Acids. They
also offer tools for use in sample preparation through to detection
for Aflatoxin and Ochratoxin analysis.
Click here to visit the Pickering
Laboratories website or contact the Chromatographic Specialties
Inc. Technical Support
Team for more information.
New Literature
Trevigen
- Product Focus Brochure 2008-09
This new brochure offers information on products including Basement Membrane Extracts, the DIVAA™ Directed in vivo Angiogenesis Assays, CometAssay™, Cell Invasion/Migration Assays, TACS™ in situ Apoptosis Detection Kits and more.
Promotions
Dualsystems
- 5% OFF All Yeast Based Screening Services
Dualsystems Biotech is the market leader for customized yeast-based screening methods. They are specialized in the discovery of protein-protein interactions.
Click here for promotion details
USB
- Save 20% OFF USB Molecular Biology Ladders
For a limited time, save 20% OFF USB's Ladders for
DNA, RNA and Proteins.
Click
here for promotion details.
USB
- Spend $150 on USB Biochemicals and
we'll send you the USB fitness pack Free!
USB
provides biochemicals that deliver reliable and consistent
results. USB biochemicals and Ultrapure brand products
meet the highest industry standards for quality. They
are available in a variety of grades such as ACS Reagent
Grade, CP Grade (Chemically Pure), USP Grade (USBioAnalyzed)
and Molecular Biology Grade.
Don't forget about their line of convenience reagents. USB offers an extensive selection of Ultrapure biochemical reagents in premixed, ready-to-use formats for convenience in any lab.
Now's a great time to step forward and purchase USB Biochemicals and convenience reagents. When you spend $150 or more, we'll send you a USB water bottle and pedometer to track your own fitness results.
Click here for promotion details.
Active Motif - Order any TransAM™ ELISA Kit and Receive a FREE Nuclear Extract Kit
TransAM™ Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell culture extracts. TransAM is rapid, non-radioactive, highly specific and 100-fold more sensitive than gel shift assays. Kits are available for analysis of over 40 transcription factors.
Click here for promotion details
Active Motif - Order ChIP-IT™ Express HT and Receive a FREE MAG-96 Magnetic Stand
ChIP-IT™ Express HT allows you to perform chromatin IP in a fast, reproducible high-throughput format. It combines the efficiency of the groundbreaking magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate format, enabling the rapid and efficient processing of a large number of ChIP reactions.
Click here for promotion details
Active Motif - Order 3 Antibodies, Get a 4th FREE!
Active Motif offers a broad line of highly-characterized antibodies directed against a variety of histones, histone modifications, transcription factors and cell signaling-related proteins. They are ideal for performing Western blots, ELISAs, ChIP, EMSA and immunocytochemistry.
Click here for promotion details
Simport
- 25% OFF LockMailer™ Microscope Slide Jar
Get a 25% Discount on
Simports new LockMailer™ Jars,
tamperproof multi purpose containers for mailing,
staining or storing microscope slides.
Click here for promotion details
Assay
Designs - Buy 3, Get 1 Free - Antibody and Protein
Promotion
Offer extended until December 31st, 2008
Fall into savings with this buy 3, get 1 free promotion on Assay Designs top selling antibodies and proteins. All you need to do is select at least 4 Assay Designs™ antibodies or proteins from the list below and you will receive the lowest priced product free of charge.
Click here for promotion details
Technical Report
Reliable extraction yielding
highly pure RNA from a variety of sample materials
by Macherey-Nagel
MACHEREY-NAGEL, a leader in purification kit innovation and manufacture, has answered the need for reliable RNA purification out of various starting materials.
NucleoSpin® RNA II is suited for fast and easy extraction of highly pure RNA out of cultured cells and various tissues like e.g. paraffin embedded tissue, biological fluids, bacterial cells (Gram-negative and Gram-positive).
High yields up to 70 µg ready-to-use RNA can be obtained.
RNA purified with NucleoSpin® RNA II is suitable for applications like real-time PCR, RT-PCR, Northern blotting, primer extension, array technology and RNase protection assays.
Filters and quantitative DNA removal for best possible results
NucleoSpin® Filters (shredders) are part of the kit and cause an efficient homogenization and reduction of viscosity.
Using the rDNase included in NucleoSpin®
RNA II the resulting RNA is without detectable genomic DNA.
In fig. 1 gel-electrophoresis results of RNA purified from five
million HeLa cells are shown. On a denaturating formaldehyde
gel (1A) the RNA shows a band pattern typical for HeLa cells,
consisting of 18S rRNA, 28S rRNA and a nuclear precursor RNA
(n.p. RNA) which is sometimes misleadingly interpreted as genomic
DNA. The high molecular weight band indicates highest RNA quality
as precursor RNA is very prone to degradation.
Fig. 1b shows the same RNA sample in a TAE gel which stains
DNA considerably stronger than denaturating formaldehyde gels.
The RNA fraction has been treated with RNAse in order to get
a clear view on possible DNA contamination in the RNA elution
fraction, which however cannot be detected.
Fig. 1: a) On the denaturating formaldehyde agarose gel the RNA eluate shows not only the 28S and 18S rRNA but as well the nuclear precursor RNA (n.p. RNA). b) Using non denaturating conditions and having digested the RNA only the degraded RNA can be detected but no chromosomal DNA.
Accurate highly sensitive results
NucleoSpin® RNA II allows the purification of highly pure RNA suitable for accurate results in demanding downstream application, like RT-PCR.
Fig. 2: Agarose gel of RT-PCR products using
different amounts of HeLa cells
In Fig. 2 a RT-PCR of total RNA isolated with the NucleoSpin® RNA II standard protocol is shown starting with 105, 104, 103, 330, 100, 33,10, 3 and 0 HeLa cells. The RT-PCR was done using 3 µl of total RNA each (elution volume 40 µl) and ß-actin primers. The specific RT-PCR product is 626 bp of size. The results show that the recovery from the NucleoSpin® RNA II is as efficient that RT-PCR is possible with only RNA from 10 HeLa cells.
Outperforming the competitors kits
Fig. 3 shows purified total RNA from 106 HeLa cells in comparison to competitor Q. PCR reactions were performed for 30 cycles using different sensitive gene-specific primers. An aliquot of the PCR reaction was analyzed using a 1% TAE agarose gel.
| Lane 1 - 4 | AFR3 Gene | 1069 bp | single copy target |
| Lane 6 - 9 | Actin 3-5 | 838 bp | multi copy target |
| Lane 11- 14 | Gamma actin | 451 bp | multi copy target |
| Lane 5 / 10 | blank | ||
| M | Marker 100 bp DNA ladder, MBI-Fermentas | ||
Fig. 3: Agarose gel of PCR products after RNA
extraction using the NucleoSpin® RNA II (MN) and a competitor
product (Q) for the detection of chromosomal DNA contamination
Even when small targets are amplified no DNA contamination can be detected using the MACHEREY-NAGEL kit whereas the kit from competitor Q shows obvious contamination resulting in false positives.
NucleoSpin® RNA II provides you best results in your sensitive downstream applications.
NucleoSpin® RNA II offers a full solution
including the DNase and filters for optimal results.
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Sample Material | < 5 x 106 cultured cells < 109 bacterial cells, up to 108 yeast cells < 30 mg tissue |
| Fragment Size | > 200 b |
| Typical Yield | 14 µg from 106 HeLa
cells 70 µg from 109 bacterial cells |
| A260/280 | 1.9-2.1 |
| Typical RIN (RNA Integrity Number) | > 9 |
| Elution Volume | 40-100 µl |
| Preparation Time | 30 min/6 preps |
| Binding Capacity | 100 µg |
Tradeshows and Events
| Tradeshow | Location | Date |
| RiboClub | Hôtel Chéribourg Orford, Qc |
September 22 and 23, 2008 |
