e-Lynx - January 2010


Dear Researcher,

We are pleased to present the January e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.

New Suppliers

Semba Biosciences – Simulated Moving Bed Chromatography Systems for Molecular Separations

The Semba Octave™ Chromatography System is a fully automated SMBC (Simulated Moving Bed Chromatography) system for research and preparative scale molecular purification. This system enables:

  • High-throughput production of proteins, antibodies, enantiomeric compounds, carbohydrates, etc.

  • Continuous purification from crude cell lysates or racemic mixtures

  • Scalable from milligrams to tons of purified product

  • Unparalleled efficiency in column usage and lower solvent consumption

  • Simple system set-up and parameter optimization

Click here to view a Video Tutorial on the SMBC technology.
Click here to read more about the Octave™ system.

 

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New Products

Macherey-Nagel – NucleoSpin® miRNA for micro RNA Extraction
Parallel isolation of small and large RNA

  • Simultaneous isolation of small RNA (<200 nt) and large RNA (>200 nt) in separate fractions

  • Additional isolation of the protein fraction – allows for confirmation of RNA results, on the protein level

  • Excellent RNA recovery without phenol/chroloform – Less hazardous

  • Compatible with a variety of sample material – Cultured cells, animal tissue, plant tissue and reaction mixtures

Click here for more information

Product Number
Description
Pkg Size
MCN74097110 NucleoSpin® miRNA 10 Preps
MCN74097150 NucleoSpin® miRNA 50 Preps
MCN740971250 NucleoSpin® miRNA 250 Preps
Macherey-Nagel – NucleoSpin® Dx Blood
Rapid purification of high-quality DNA from blood

  • Compatible with common blood collection tubes and anticoagulants

  • Suitable for use with fresh or frozen samples

  • Reproducible results for reliable downstream applications
Click here for more information.

Product Number
Description
Pkg Size
MCN74089950 NucleoSpin® Dx Blood 50 Preps
MCN740899250 NucleoSpin® Dx Blood 250 Preps
Platypus Technologies – Oris™ Pro Cell Migration Assay

The Oris™ Cell Migration Assay is designed with a unique cell seeding stopper, detection mask, and stopper tool. This unique plate design generates highly reproducible results using a microscope, digital imaging system or fluorescence plate reader.

The new Oris™ Pro Cell Migration Assay enables the use of automated liquid handling equipment for cell seeding and allows unrestricted access to cells throughout the experiment.

Click here for more information.

Product Number
Description
Pkg Size
PPPROCMA1 Oris™ Pro Cell Migration Assay 1 Pack
PPPROCMA5 Oris™ Pro Cell Migration Assay 5 Pack
PPPROCMACC1 Oris™ Pro Cell Migration Assay – Collagen Coated 1 Pack
PPPROCMACC5 Oris™ Pro Cell Migration Assay – Collagen Coated 5 Pack
Expedeon – RunBlue Pre-cast Gels
Now available in an 8 x 10 cm Mini-Gel Format

RunBlue gels are now available in an 8 x 10 cm format for use in common electrophoresis units

  • 2 Year Shelf Life - Never throw gels away again!

  • Up to 10x Stronger than other gels to prevent tearing

  • Load up to 35 microlitres of sample per lane

  • Stacking gel dyed red for ultimate ease of loading

  • Fingers extend above lower front plate eliminating flow-over from one well to another

  • No comb or tape to remove, simply take it from its individually sealed packet and use
Product Number
Description
Pkg Size
XPBCG42012 SDS Gel 4-20% (8 x 10cm) 12 well 10
XPBCG01212 SDS Gel 12% (8 x 10cm) 12 well 10

Check out our Promotions Section for learn more about how you could receive and iPod when purchasing Expedeon Pre-cast Gels.

NuGEN – Ovation® RNA-Seq System for Next Gen Sequencing

The Ovation RNA-Seq System provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications (transcriptome sequencing).

  • Low input – Comprehensive transcriptome coverage from 500 pg of total RNA

  • Fast – Streamlined procedure without poly(A) enrichment in 6 hours

  • Easy – Simple integration into standard Next Gen Sequencing platforms

Click here for more information.

Product Number
Description
Pkg Size
NU710008 Ovation® RNA-Seq System 8 rxns
Heathrow Scientific – New Laboratory Storage Products
20-Place Slide Tray Rack
Holds 5 Heathrow 20 place slide trays
Tall Slide Box – 100 Place
Designed for storage of non-standard microscope slides
True North Slide Box
Protect your slides in the ultra low temperature ranges – down to -80 °C
15 & 50 mL Tube Storage Box
Both the 15 & 50 mL tube grids are included with each box
Rota-Rack® Duo
Work with PCR tubes and Centrifuge tubes side by side
Droplet™ Phloebotomy Tray Accessories
Urine Collection Insert, Side Pockets and Lid
Cargo Manual Pipette Rack
Ideal for serological pipettes; securely connect racks together.

 

Product Number  Description Pkg Size
HS99040A 20-Place Slide Tray Rack Each
HS15979A Tall Slide Box – 100 Place Each
HS15988H True North Slide Box Each
HS23271A 15 & 50 mL Tube Storage Box 5
HS29041A Rota-Rack® Duo Each
HS2201C Droplet™ Urine collection insert 6
HS2203 Droplet™ Side Pockets 2
HS2202 Droplet™ Lid Each
HS20615M Cargo Manual Pipette Rack 3

 

 

Assay Designs – uPlex and PathPlex MultiBead Kits

The MultiBead assay technology relies on the ability of flow cytometers to separate beads covalently coupled to antibodies based on their size and fluorescence intensity. MultiBead assays can simultaneously quantify up to 36 analytes in a single well by utilizing multiple bead sizes each with different fluorescence intensities.

With the uPlex MultiBead kits, you can create singleplex or multiplex kits to meet your specific research needs.
Below is a complete list of analytes to choose from for custom uPlex kits.

IFN-γ IL-5 IL-15 MIP-1β
IL-1α IL-6 IL-17A PGE2
IL-1β IL-8 IL-21 RANTES
IL-2 IL-10 IL-29 TNF-α
IL-4 IL-13 MCP-1 TXB2

Pathplex MultiBead multiplex panels combine analytes that are used in studying a specific pathology or pathway.

Product Number
Description
Analytes
ADI980003 Tumorigenic (human) Pathplex MultiBead Panel IFN-γ, IL-2, IL-4, IL-6, IL-8, IL-10, MCP-1, PGE2, TNF-α
ADI980005 Th1/Th2 (human) Pathplex MultiBead Panel IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-α
ADI980006 Respiratory (human) Pathplex MultiBead Panel IFN-γ, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, IL-15, IL-17, MCP-1, RANTES
ADI980007 Pain (human) Pathplex MultiBead Panel IFN-γ, IL-1β, IL-6, IL-8, IL-10, PGE2, TNF-α, TXB2
ADI980008 Cardiovascular (human) Pathplex MultiBead Panel IL-1α, IL-1β, IL-6, IL-8, MCP-1, PGE2, TNF-α, TXB2

Click here to view videos of the MultiBead kit in Action.
Please contact our Technical Support Team for more information.

Enzo Life Sciences – Nuclear-ID™ Blue/Green Cell Viability Reagent

The reagent is a mixture of two DNA dyes: a cell-permeable, blue fluorescent dye and a cell-impermeable, green fluorescent dye that is able to enter dead cells with compromised membrane integrity. The staining pattern permits determination of the ratio of live-to-dead cell populations by fluorescence/confocal microscopy. The dye leaves the cytoplasm unstained, potentially allowing visualization of other cell markers.

Click here for product details.

Product Number
Description
Pkg Size
ENZ53004C100 Nuclear-ID™ Blue/Green Cell Viability Reagent 100 µL

 

Micronic – SampleScan Plus Single Tube Barcode Reader
  • Reads 1D and 2D barcodes

  • Keyboard wedge functionality - data automatically transfers to any open program on your PC

  • Scans and decodes in less than one second

  • Black tubeholder around scanner lens can be removed to read 1D barcodes or non-round tubes

  • One USB connection

  • No power supply needed

  • Very small footprint: 4”w x 4”d x 5.5”h

 

Product Number
Description
Pkg Size
MSMP85220 SampleScan Plus Each

Click here for more information and introductory pricing.

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Promotions

Greiner Bio-One – The Free Pizza Party Promotion is Back !
Get it now before it's all gone

For the cold Canadian winter months of January to March 2010 - enjoy hot, delicious pizza on us!

Accumulate $500 in Purchases of Greiner Bio-One Products from MJSBioLynx and Receive a $50 Pizza Gift Certificate.
Greiner Bio-One offers high quality labware such as cell culture labware, microplates and tubes.

Click here for more information.

Expedeon – FREE iPods and iTunes with the Purchase of RunBlue Pre-cast Gels

Until March 31st, 2010, receive a FREE gift when you purchase 3 or more Boxes of 10 RunBlue Pre-Cast Acrylamide Gels

3 Boxes = FREE iPod Shuffle
6 Boxes = FREE iPod Shuffle + $40 iTunes Vouchers
9 Boxes = FREE iPod Nano + $40 iTunes Vouchers

Click here for more information.

Simport - Buy One set of Cryovials and receive 50% off a second set

Buy one case of Simport Cryovials between December 1st 2009 and March 31st 2010 and get 50% off a second case*.
Also, complete and return the request slip from the pdf download to receive a free gift. **

Click here to read more.

*Second product to be of equal or lesser value than the first
**Gift includes - tube rack, storage box, a workstation rack and assorted capinserts

Micronic – Introductory Pricing on the SampleScan Plus Single Tube Barcode Reader.

Introductory pricing is currently in available until February 26, 2010
Click here for details.

 

 

 

Product Number
Description
Pkg Size
Promo Price
MSMP85220 SampleScan Plus Each $2666

 

USB - Express Yourself! - Purchase $375 of USB Products and Receive a FREE Customized T-Shirt

Offer extended until March 31st, 2010

Create your one-of-a-kind T-shirt online by answering six defining questions, and in a few weeks, you’ll receive your personalized T-shirt that expresses the true you.

USB Products Include:

  • PCR/qPCR/RT-PCR - Nucleotides - ExoSAP-IT®
  • Molecular Biology Enzymes and Kits - RNA Analysis
  • Biochemicals and Reagents

Click here for promotion details.

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Events

Tradeshows
Meet MJSBioLynx at the following events:
Tradeshow Date
Minishow – Université de Montréal
Pavillon Jean-Coutu - Mezzanine
 January 27, 2010
10 :00 am to 2 :00 pm

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Technical Report

Microplates for Enzyme Linked Immunosorbent Assays (ELISA) – by Greiner Bio-One

ELISA (Enzyme Linked Immunosorbent Assay) is one of the most widely used biochemical method in laboratory analysis and diagnostics. Analytes such as peptides, proteins, antibodies and hormones can be detected selectively and quantified in low concentrations among a multitude of other substances. Additionally, ELISAs are rapid, sensitive, cost effective and can be performed in a high-throughput manner. An ELISA is used in a vast variety of different types of assays (e.g. direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA, see technical
appendix). Nevertheless, all ELISA variants are based on the same principle, the binding of one assay component – antigen or specific antibody – to a solid surface and the selective interaction between both assay components. Molecules not specifically interacting with the assay
component bound to the solid surface are washed away during the assay. For the detection of the interaction the antibody or antigen is labelled or linked to an enzyme (direct ELISA). Alternatively, a secondary antibody conjugate can be used (indirect ELISA). The assay is developed by adding an enzymatic substrate to produce a measurable signal (colorimetric, fluorescent or luminescent). The strength of the signal indicates the quantity of analytes in the sample.

Click here to read more.

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