e-Lynx - July 2010

Dear Researcher,

We are pleased to present the July e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.

New Products

Greiner Bio-One - Advanced TC Cell Culture Labware
  • Modified growth surface for improved assay consistency

  • Permits usage of serum-reduced or serum-free media

  • Provides optimal cultivation conditions for sensitive cells

  • Improves cell adherence and yield

  • Available in multi-well plates, microplates, culture flasks and dishes
Click here for more information

Greiner Bio-One - Cryo.S Tubes with Datamatrix

Greiner Bio-One now offers a Datamatrix coding service for their Cryo.S cryogenic storage tubes.

  • Availability of four different tube sizes: 1mL or 2 mL with internal threads and 2mL or 4mL with external threads

  • Virtually any kind of information such as sequential sample IDs or characters can be encoded

  • High data capacity and strong resistance to mechanical and chemical damage

Click here for more information.
Please contact our Technical Support Team to set up your Cryo.S tubes with datamatrix.

NuGEN - Sample Preparation for Next Generation Sequencing and DNA Library Production

Ovation® 3'-DGE System
Offers rapid eukaryotic expression profiling and the technical advantages of NGS with the convenience, throughput and cost of microarrays

  • Generate micrograms of double stranded cDNA from 10 ng - 100 ng of purified RNA

  • Simple, fast, automatable workflow

  • Compatible with all leading NGS platforms

Click here for more information.

Encore™ NGS Library and Multiplex Systems

  • Provide simple, fast, automatable workflow for library construction in as little as three hours, with only two purification steps and no gel extraction required
  • Cost effective and scalable - Optional indexing capability for multiplex sequencing up to eight samples to improve sample throughput and reduce sequencing costs

  • Designed for use with the Ovation RNA-Seq System or the Ovation 3'-DGE System for library construction in 9-10 hours

Click here for more information.

Product Number
Description
Pkg Size
NU7200 Ovation 3'-DGE System 8 Reactions
NU300 Encore NGS Library System I 8 Reactions
NU301 Encore NGS Multiplex System I 32 Reactions
Enzo Life Sciences - SUMO-Qapture™ Isolation and Enrichment of SUMOylated Proteins

SUMO-Qapture kits facilitate the affinity purification of SUMOylated proteins from cell extracts and tissue lysates using appropriate SUMO interacting motif (SIM)-containing affinity matrices. Click on the links in the table below for more information.

Product Number
Description
Pkg Size
ENZUW10000001 SUMO-Qapture-T kit Kit
ENZUW09550001 PolySUMO-Qapture kit Kit
Enzo Life Sciences - eFluxx-ID™ Multidrug Resistance Assay Kits

eFluxx-ID™ Multidrug resistance assay kits profile ABC transporter protein activity in cells, detecting and distinguishing among all three major multidrug resistance proteins: MDR1 (P-glycoprotein), MRP1/2 and BCRP. The kits feature a simple and reproducible mix-and-read protocol optimized for flow cytometry.

Click here for more information.

Product Number
Description
Pkg Size
ENZ51029K100 eFluxx-ID™ Green Multidrug resistance assay kit 100 Assays
ENZ51030K100 eFluxx-ID™ Gold Multidrug resistance assay kit 100 Assays
Enzo Life Sciences - Cell-ID™ Autophagy Detection Kit

The Cell-ID™ Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy and flow cytometry. The detection reagent displays bright green fluorescence in vesicles produced during autophagy and has been validated under a wide range of conditions known to modulate autophagy pathways.

Click here for more information.

Product Number
Description
Pkg Size
ENZ51031K200 Cell-ID™ Autophagy Detection Kit 200 Assays

Figure: HepG2 cells were incubated in EMEM medium supplemented with 10% fetal bovine serum (left) or in Earl’s buffer (right, starved). Starvation induces a 2.5-fold increase in fluorescence intensity.

Assay Designs - Assay Blaster! Data Analysis Software
  • Simplified data analysis

  • Adjust parameters and settings to your needs

  • Optionally use duplicate or triplicate data series and remove outliers

  • Analyze mean signal, net signal, or %B/Bo results

  • Multiple curve fit options, including point-to-point, 4PL, and 5PL

Click here for more information.

Product Number
Description
Pkg Size
ADI280002 Assay Blaster! Data Analysis Software Each
USA Scientific - ErgoOne™ Pipettors

ErgoOne™ pipettors combine researcher requested features with the latest ergonomic principles to provide optimum performance. Maintain maximum control while minimizing repetitive strain.

Product Number
Description
Pkg Size
US71000125 0.1-2.5 μl Single Channel Pipettor Each
US71000510 0.5-10 μl Single Channel Pipettor Each
US71000221 2-20 μl Single Channel Pipettor with ultra micro tip cone Each
US71000220 2-20 μl Single Channel Pipettor with standard tip cone Each
US71001100 10-100 μl Single Channel Pipettor Each
US71002200 20-200 μl Single Channel Pipettor Each
US71001000 100-1000 μl Single Channel Pipettor Each
US71005000 500-5000 μl Single Channel Pipettor Each

Click here for more information.

Promotions

Micronic - Save 30% off the QuickGene Software for DNA analysis

QuickGene is a new molecular biology software for DNA analysis, assembly and cloning. It contains a complete and up-to-date database of restriction enzymes with recommended buffers, temperatures, methylation sensitivity, etc.

Save 30% on the QuickGene software until July 31st, 2010. Click here for details.

Cannot be combined with other discounts or offers.

Greiner Bio-One - Buy 3, Get 1 Free on ELISA Plates and PCR Plates

Top-quality, competitively-prices ELISA and PCR plates now at additional savings:
Buy 3 cases, get 1 FREE - Until November 30th, 2010

Click here for details.

Simport - Special Pricing on Tamper Evident Labware

Protect your sample's integrity!
Simport tamper-evident security closures provide an immediate indication if tubes, vials or containers have been opened, pilfered and/or tampered with.

From June 1st to August 31st, 2010
Take advantage of special pricing on a wide selection of Tamper Evident Labware
Click here for details.

PromoKine - 15% Discount on all Products for Cell Analysis and Apoptosis Research

This offer refers to all products of chapter 1 & 2 of the PromoKine Cell Biology Catalogue
(i.e. all products with Cat.No. PK-CA***).

If requested, you will also get a free sample of the Colorimetric Cell Viability Kit I (WST-8).

Cell Analysis Apoptosis

Offer valid until August 31, 2010
Click here for details

Sunrise Science Products - 3 Special Offers on Yeast Media
1) Save 20% on any amino acid mixture and get FREE sterile plating beads!

Choose any Complete Supplement Mixture (CSM), Synthetic Complete Supplement Mixture (SC), Hollenberg Supplement Mixture (HSM) or Brent Supplement Mixture (BSM) from Sunrise's extensive list and get EZ-Plate beads (# SN3001000) for free! - Please reference Promo Code AA20EZ0.

2) Save 20% on YNB+Nitrogen

Vitamins, minerals, trace elements and nitrogen all in one powder!
Product Number SN1501250 - Please reference Promo Code YNBN20.

3) Save 15% on Yeast Culture Grade Agar

Eliminate variations in plating conditions caused by inferior agar!
Product Number SN19151KG - Please reference Promo Code AGAR15.

Offer valid until September 15, 2010
Click here for more information.

Micronic - 3 Special Offers on Sample Tracking Systems

1) Purchase any Micronic 2D Barcode Reader and receive one FREE Starter Edition of the SampleWare database management software. Click here for details.

2) Special Pricing - Purchase the SampleWare laboratory mangement software version 3.1 starter edition for only $115. Click here for promotion details. (cannot be combined with other offers or discounts)

3) Starter Pack Plus - Includes 2D barcoded tubes, SampleWare software, SampleScan Plus barcode reader and decapper, all at a special price. Click here for details.

Offers valid until September 30th, 2010.

Meridian Life Science - Introductory Pricing on the New Lyophilized Mouse IgG

Meridian Life Science manufactures large quantities of Mouse IgG in a variety of formulations for use as a Human Anti-Mouse Antibody (HAMA) blocker in immunoassays. To meet and exceed the product requirements of customer specifications and applications, they have now developed a Lyophilized Mouse IgG Protein.

Until July 30, 2010, purchase this product at a Special Introductory Price of $308.70!

Product Number
Description
Pkg Size
BDA66185MLY Lyophilized Mouse IgG 1g

Cannot be combined with other promotional offers.

StressMarq Biosciences - 50% OFF Selected Antibodies

For a limited time, MJSBioLynx Inc. is offering up to 50% off the following products:

Product Number
Description
Pkg Size
50% Discount until September 30, 2010
SMSMC175C HDEL, 2E7, Monoclonal Antibody 25 µg
SMSMC175D HDEL, 2E7, Monoclonal Antibody 100 µg
50% Discount from July 26 to 30, 2010
SMSMC178C Synaptophysin, EP10, Monoclonal Antibody 25 µg
SMSMC178D Synaptophysin, EP10, Monoclonal Antibody 100 µg
SMSMC179C Anti-VAMP-1,2, Monoclonal Antibody 25 µg
SMSMC179D Anti-VAMP-1,2, Monoclonal Antibody 100 µg
SMSMC180C Anti-SNAP-25, SP-12, Monoclonal Antibody 25 µg
SMSMC180D Anti-SNAP-25, SP-12, Monoclonal Antibody 100 µg
SMSMC181C Anti-Syntaxin, SP-6, Monoclonal Antibody 25 µg
SMSMC181D Anti-Syntaxin, SP-6, Monoclonal Antibody 100 µg

Cannot be combined with other discounts or promotions.
Please contact our Technical Support Team for additional information.

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Events

Tradeshows
Meet MJSBioLynx at the following events:
Tradeshow Date
RiboWest 2010
University of Lethbridge, AB
 July 11 - 14, 2010

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Technical Report

Comparison of the Oris™ Cell Migration Assay to the Scratch Assay
by Platypus Technologies

Cell migration is integral to many physiological processes, including embryonic development, tissue regeneration, and wound healing. In
addition, cell migration is involved in tumor metastasis and atherosclerosis(1). One assay commonly used to study cell migration in vitro is the scratch assay. The scratch assay is performed by creating a cell-free gap, or “scratch”, on a confluent cell monolayer upon which cells at the edge of the opening move inward to close the scratch. Cell migration can be assessed by comparing images captured at the onset of the scratch creation and at user-defined intervals during scratch closure. The scratch assay is straightforward to perform and is inexpensive. However, methods for creating the scratch vary from lab to lab and results can be highly variable. Furthermore, the process of scratch formation has been shown to damage the underlying extracellular matrix (ECM)(2).

The Oris™ Cell Migration Assay (CMA) was designed to address the limitations of the scratch assay. Click here to read more.

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Announcement

New Technical Sales Representative for Central and Southwestern Ontario

MJSBiolynx Inc. is pleased to announce the appointment of Ms. Salma Al-Shehabi as the Technical Sales Representative for Central and Southwestern Ontario.

Salma holds an Honours Bachelors of Science with specialization in Biochemistry from the University of Ottawa. She successfully concluded an internship at Lethbridge Research Centre, Agriculture Agri-Food Canada (LRC-AAFC) focusing on biodiversity in compost. Salma complements her science background with over six years experience in various roles in teaching, administration and sales.

Salma will be able to visit you at your convenience to learn more about your research and discuss any of your research needs. She can be reached through our Brockville office toll free at 1-888-593-5969 or by e-mail at salmaa@sales.biolynx.ca

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