e-Lynx - March 2010


Dear Researcher,

We are pleased to present the March e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.


New Products

Micronic - Biomatrica DNAgard - Room Temperature Storage of DNA in Tissues and Cells

  • Stabilize DNA immediately in complex samples (animal tissue and cells)

  • Submerged samples are stable for at least 2 months at room temperature

  • Unique optional dry-down method provides convenient long-term stability for one year

  • Reduced sample shipping costs - No dry ice required

  • Ideal for field collection and tissue harvesting
Product Number
Description
Pkg Size
MSMB10008 DNAgard Trial Kit 3 x 500 µL
MSMB10009 DNAgard 50 mL
MSMB10010 DNAgard 100 mL
MSMB10011 DNAgard Barcoded Tubes, 2mL with Screw caps 100
MSMB10012 DNAgard Tubes (empty) 25
MSMB10013 DNAgard 96-Well Plates 5

 

Micronic - Biomatrica PCRboost Reagent

PCRboost is a reagent that enhances end-point and reverse transcription-PCR performance fivefold or more by improving sensitivity and specificity during amplification of genomic DNA or RNA templates

  • Greatly improves amplification of difficult samples - Including trace samples, degraded samples, low-copy transcripts and samples containing inhibitory factors

  • Enhances yield, specificity and consistency

  • No changes to end-point or reverse transcription PCR protocols - Simply replace the water in the reaction with PCRboost

  • Saves time by reducing the number of reaction cycles
Product Number
Description
Pkg Size
MSMB10029 PCRboost Trial Kit 150 µL
MSMB10030 PCRboost Kit 1 mL
MSMB10031 PCRboost Kit 10 mL

 

Micronic - Biomatrica STRboost Reagent
Improved STR (Short Tandem Repeat) Multiplexing Performance

  • Increases peak heights and facilitates identification of individuals from picogram levels of DNA

  • Increases the number of allele calls

  • Overcomes inhibitory factors

  • Compatible with commercially available genetic identification kits such as ABI's Identifiler® and Promega's PowerPlex® 16 systems

  • Sample types include: Limited samples (trace samples, low copy number), degraded samples, blood samples contains inhibitory factors such as heme
Product Number
Description
Pkg Size
MSMB10032 STRboost Trial Kit 5 Reactions
MSMB10033 STRboost Kit 50 Reactions
MSMB10034 STRboost Kit 300 Reactions

Click here for more information on the Micronic line of Biomatrica products.


NuGEN - Ovation PicoSL WTA System - Sample Preparation for Microarray Analysis

The Ovation PicoSL WTA System provides a sensitive, rapid and simple method for preparing amplified cDNA from a range of 500 picograms to 50 nanograms of total RNA in less than five hours. The Ovation PicoSL WTA System is optimized for output at 2-4 µg.

The amplified product of the Ovation PicoSL WTA System is optimized for analysis on Affymetrix® Array Plates, Agilent Gene Expression microarrays and Illumina Genome-Wide Expression BeadChips utilizing the appropriate NuGEN® labeling modules and protocols.

 

Product Number
Description
Pkg Size
NU331048 Ovation PicoSL WTA System 48 Reactions

Click here for more information.


Trevigen - HT PARP in vivo Pharmacodynamic Assay II

Trevigen offers a validated assay, with higher sensitivity and pre-coated antibody plates to measure the effectiveness of PARP inhibitors in cell and tissue lysates for anticancer drug screening.

The HT PARP in vivo Pharmacodynamic Assay II is a high throughput, Chemiluminescent, PAR ELISA assay kit that has been validated on human peripheral blood lymphocytes and also shown to work with normal and tumor tissue. This improved assay features:

  • Pre-coated capture antibody plates.

  • Broad linear dynamic range to 1,000 pg/ml.

  • High signal to noise ratio.

  • Detection sensitivity of 2 pg/ml of PAR.

The amount of PAR present correlates directly with the activity of the PARP enzyme in cell lysates. This assay is ideal for quantification of PAR in peripheral blood mononuclear cells, tissues and cultured cells.

Product Number
Description
Pkg Size
TV4520096K HT PARP in vivo Pharmacodynamic Assay II 96 Tests

Please contact our Technical Support Team for more information.


Knauer - Bioline Biochromatograpy Systems

Our sister company, Chromatographic Specialties Inc., offers the KNAUER LC systems for biochromatography. These systems use sophisticated instrument and software solutions to achieve high quality separations.

  • The Bioline Start System is fast and accurate, particularly suited for the quantification of proteins. The Start System can also be upgraded with an autosampler for high sample throughput.

  • Bioline high resolution glass columns offer pressure stability up to 100 bar and a thermostatic jacket for temperatures between 0°C and 60°C, making them suitable for a wide range of biochromatography applications.

Click here for more information.

Knauer products are available through our sister company, Chromatographic Specialties Inc. Please contact their Instrumentation Team for more information.


Simport – CoreDish
Multiple Biopsy Containers Half Prefilled with 10% Formalin

  • Ideal for holding and transporting biopsies
  • Multi-compartment container in the shape of a dish

  • Half prefilled with 10% Neutral Buffered Formalin

  • Supplied with a leakproof closure with O-ring ensuring total protection of contents

  • Measures only 15 x 95 mm in diameter

  • Each compartment is clearly identified to allow proper placement and visualization of the biopsy being inserted

  • Eliminates the need to use a multitude of individual containers, thereby reducing risks of confusion

Click here for more information


Labnet - MultiGene Mini Compact Thermal Cycler

The MultiGene Mini is a compact and lightweight thermal cycler, but dont let its small size fool you. This cycler is packed with features typically found only on larger thermal cyclers.

  • Fast ramping - up to 5ºC/second

  • Intuitive graphical programming

  • Lightweight and compact

  • Interchangeable blocks - 24 x 0.2 ml and 18 x 0.5 ml

  • Economical - half the price of a full size unit

Click here for more information.


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New Literature

Enzo Life Sciences - Topix Newsletter - Inflammation and Cellular Stress

This issue of the Topix Newsletter contains information on Stress and Heat Shock products, Proteinase Activated Receptors (PARs), Eicosanoid Assay Kits, ImmunoSet™ ELISA Development Sets and more. Click here to view online or click here request a printed copy.


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Promotions

Promokine - Great Discounts on Transfection Reagents and Magnet Assisted Transfection Products

The PromoFectin Transfection Reagents are ideally suited for highly efficient delivery of nucleic acids (e.g. plasmid DNA, oligonucleotides, siRNA) and other biomolecules into a variety of cell types.

The Magnet-Assisted Transfection (MATra) technology is a very innovative approach to transfect numerous cell lines and primary cells with high efficiencies.

Until May 31st, 2010, Get a 15 % Discount on all PromoFectin and MATra Transfection Reagents and a 30 % Discount on the MATra Universal Magnet Plate (8x13cm plate)

Click here for promotion details.
Click here for more information about PromoFectin and MATra products.


Don't Break My Heart - Get Cardiac Cells from PromoCell instead!

PromoCell offers high quality cells from the cardiac system including; Human Cardiac Myocytes, Human Cardiac Fibroblasts, and Human Microvascular Cardiac Endothelial Cells.

Until April 30, 2010 get a FREE bottle of specialized, high quality medium with your purchase of cardiac cells and save 30% OFF the PromoCell 3D-Angiogenesis Assay.

Click here to read more.


G-Biosciences - 10% Discount on the Red 660™ Protein Assay

The RED 660™ Protein Assay produces perfect linear standard curves when compared to other protein assays. The single, ready-to-use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Until March 31st, 2010, Save 10% Off the Red 660™ Protein Assay.

Click here for promotion details.


Greiner Bio-One – The Free Pizza Party Promotion is Back !
Get it now before it's all gone

For the cold Canadian winter months of January to March 2010 - enjoy hot, delicious pizza on us!

Accumulate $500 in Purchases of Greiner Bio-One Products from MJSBioLynx and Receive a $50 Pizza Gift Certificate.
Greiner Bio-One offers high quality labware such as cell culture labware, microplates and tubes.

Click here for more information.

 


Expedeon – FREE iPods and iTunes with the Purchase of RunBlue Pre-cast Gels

Until March 31st, 2010, receive a FREE gift when you purchase 3 or more Boxes of 10 RunBlue Pre-Cast Acrylamide Gels

3 Boxes = FREE iPod Shuffle
6 Boxes = FREE iPod Shuffle + $40 iTunes Vouchers
9 Boxes = FREE iPod Nano + $40 iTunes Vouchers

Click here for more information.


Simport - Buy One set of Cryovials and receive 50% off a second set

Buy one case of Simport Cryovials between December 1st 2009 and March 31st 2010 and get 50% off a second case*.
Also, complete and return the request slip from the pdf download to receive a free gift. **

Click here to read more.

*Second product to be of equal or lesser value than the first
**Gift includes - tube rack, storage box, a workstation rack and assorted capinserts


USB - Express Yourself! - Purchase $375 of USB Products and Receive a FREE Customized T-Shirt

Offer extended until March 31st, 2010

Create your one-of-a-kind T-shirt online by answering six defining questions, and in a few weeks, you’ll receive your personalized T-shirt that expresses the true you.

USB Products Include:

  • PCR/qPCR/RT-PCR - Nucleotides - ExoSAP-IT®
  • Molecular Biology Enzymes and Kits - RNA Analysis
  • Biochemicals and Reagents

Click here for promotion details.

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Events

Tradeshows
Meet MJSBioLynx at the following events:
Tradeshow Date
Minishow - Children's Hospital, Montreal, QC

March 17th, 2010
10 :00 am to 12 :00 pm

Minishow - Institute Armand Frappier - Quebec, QC

March 30th, 2010
10 :00 am to 2 :00 pm

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Technical Report

WT-Ovation™ One-Direct RNA Amplification System Performance
by NuGEN Inc.

Biological systems are typically composed of heterogeneous cell populations that collectively determine the physiological state of the organism or biome. Determining the functional contribution of distinct cells in such a complex matrix requires the granularity of a true single-cell interrogation methodology.

Traditional gene expression studies use RNA isolated from a large number of cells, and therefore inherently average effects across the population. Single-cell differences in gene expression levels are not observed. For example, it is critical to consider cell-to-cell differences in expressed genes when studying blood-borne tumor cells, activated leukocytes, and cells involved in early-stage development. Moreover, the inherent inefficiency of traditional RNA isolation and purification processes limits their usefulness when working with samples consisting of small numbers of cells.

The WT-Ovation™ One-Direct System is based on the proven Ribo- SPIA® amplification process. This system makes microarray analysis possible with material from just a few cells, or even one cell, while eliminating the requirement for RNA isolation and purification steps. Amplification from cell lysates without additional purification steps is a key technological breakthrough that eliminates the sample-consuming steps of RNA isolation and purification and enables researchers to work with just a few isolated cells, allowing them to access their smallest, most challenging samples.

Click here to read more.

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